Spatial heterogeneity of bone marrow endothelial cells unveils a distinct subtype in the epiphysis.
Journal
Nature cell biology
ISSN: 1476-4679
Titre abrégé: Nat Cell Biol
Pays: England
ID NLM: 100890575
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
08
11
2022
accepted:
28
07
2023
medline:
1
11
2023
pubmed:
6
10
2023
entrez:
5
10
2023
Statut:
ppublish
Résumé
Bone marrow endothelial cells (BMECs) play a key role in bone formation and haematopoiesis. Although recent studies uncovered the cellular taxonomy of stromal compartments in the bone marrow (BM), the complexity of BMECs is not fully characterized. In the present study, using single-cell RNA sequencing, we defined a spatial heterogeneity of BMECs and identified a capillary subtype, termed type S (secondary ossification) endothelial cells (ECs), exclusively existing in the epiphysis. Type S ECs possessed unique phenotypic characteristics in terms of structure, plasticity and gene expression profiles. Genetic experiments showed that type S ECs atypically contributed to the acquisition of bone strength by secreting type I collagen, the most abundant bone matrix component. Moreover, these cells formed a distinct reservoir for haematopoietic stem cells. These findings provide the landscape for the cellular architecture in the BM vasculature and underscore the importance of epiphyseal ECs during bone and haematopoietic development.
Identifiants
pubmed: 37798545
doi: 10.1038/s41556-023-01240-7
pii: 10.1038/s41556-023-01240-7
pmc: PMC10567563
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1415-1425Subventions
Organisme : Ministry of Education, Culture, Sports, Science and Technology (MEXT)
ID : 18H05042, 18K19553, and 19H03397
Organisme : Japan Agency for Medical Research and Development (AMED)
ID : 21gm6210017h0003 and 22gm6210017h0004
Organisme : MEXT | Japan Science and Technology Agency (JST)
ID : JPMJMS 2024
Informations de copyright
© 2023. The Author(s).
Références
Nature. 2018 Feb 22;554(7693):475-480
pubmed: 29443965
EMBO J. 2013 Jan 23;32(2):219-30
pubmed: 23188081
Dev Cell. 2014 May 12;29(3):330-9
pubmed: 24823376
Theranostics. 2020 May 1;10(13):5957-5965
pubmed: 32483430
Nature. 2019 May;569(7755):222-228
pubmed: 30971824
Nature. 2014 Jan 16;505(7483):327-34
pubmed: 24429631
J Clin Invest. 2016 Apr 1;126(4):1471-81
pubmed: 26950423
Nature. 2014 Mar 20;507(7492):323-328
pubmed: 24646994
Front Neural Circuits. 2019 May 08;13:29
pubmed: 31133819
Science. 2017 Aug 25;357(6353):
pubmed: 28775214
Nature. 2016 Jan 21;529(7586):316-25
pubmed: 26791722
Cell. 1997 May 30;89(5):747-54
pubmed: 9182762
Nature. 2014 Apr 24;508(7497):536-40
pubmed: 24590069
Cell. 2011 Sep 16;146(6):873-87
pubmed: 21925313
Cell. 2020 Feb 20;180(4):764-779.e20
pubmed: 32059779
Am J Pathol. 2013 Nov;183(5):1559-1570
pubmed: 24012677
Cell. 2005 Jul 1;121(7):1109-21
pubmed: 15989959
Nat Med. 2014 Nov;20(11):1270-8
pubmed: 25282358
Cell. 1997 Nov 28;91(5):661-72
pubmed: 9393859
Nature. 2000 Mar 9;404(6774):193-7
pubmed: 10724173
Nature. 2013 Oct 31;502(7473):637-43
pubmed: 24107994
Nat Rev Mol Cell Biol. 2019 May;20(5):303-320
pubmed: 30745579
J Clin Invest. 2007 Jun;117(6):1616-26
pubmed: 17549257
Cell. 2014 Oct 23;159(3):584-96
pubmed: 25417109
Bonekey Rep. 2014 Oct 15;3:578
pubmed: 25328674
Cell. 2019 Jun 13;177(7):1915-1932.e16
pubmed: 31130381
Nature. 2012 Mar 18;484(7392):110-4
pubmed: 22426001
J Exp Med. 2022 Apr 4;219(4):
pubmed: 35319724
Dev Cell. 2010 Aug 17;19(2):329-44
pubmed: 20708594
FEBS Lett. 2000 Mar 31;470(3):263-8
pubmed: 10745079
Ann N Y Acad Sci. 2007 Jun;1106:41-53
pubmed: 17332071
Cell. 2002 Jan 11;108(1):17-29
pubmed: 11792318
J Am Acad Orthop Surg. 2010 Nov;18(11):676-86
pubmed: 21041802
Exp Anim. 2010;59(5):615-22
pubmed: 21030789
J Exp Med. 2008 Oct 27;205(11):2507-13
pubmed: 18824583
Nature. 2018 Nov;563(7730):254-258
pubmed: 30401834
Nat Commun. 2020 Dec 9;11(1):6314
pubmed: 33298956
Nature. 2016 Apr 21;532(7599):323-8
pubmed: 27074509
Nat Med. 2018 Jun;24(6):823-833
pubmed: 29785024
Cell Stem Cell. 2009 Mar 6;4(3):263-74
pubmed: 19265665
Nature. 2016 Apr 21;532(7599):380-4
pubmed: 27074508
Nature. 2014 Mar 20;507(7492):376-380
pubmed: 24647000
J Hered. 1976 Jan-Feb;67(1):11-18
pubmed: 1262696
Cell Stem Cell. 2019 Dec 5;25(6):768-783.e6
pubmed: 31761723
Nature. 2019 Mar;567(7747):234-238
pubmed: 30814736
Hum Genet. 2021 Aug;140(8):1121-1141
pubmed: 34169326
Int Immunol. 2002 Jun;14(6):637-45
pubmed: 12039915