A mutant methionyl-tRNA synthetase-based toolkit to assess induced-mesenchymal stromal cell secretome in mixed-culture disease models.
Azidonorleucine (ANL)
Bioorthogonal non-canonical amino acid tagging (BONCAT)
CRISPR/Cas9
Cell-type specific proteomics
Induced-MSCs (iMSCs)
Mesenchymal stromal cells (MSCs)
Mixed-culture disease models
Mutant methionyl-tRNA synthetase (MetRSL274G)
Secretome
Journal
Stem cell research & therapy
ISSN: 1757-6512
Titre abrégé: Stem Cell Res Ther
Pays: England
ID NLM: 101527581
Informations de publication
Date de publication:
05 10 2023
05 10 2023
Historique:
received:
20
04
2023
accepted:
25
09
2023
medline:
9
10
2023
pubmed:
6
10
2023
entrez:
5
10
2023
Statut:
epublish
Résumé
Mesenchymal stromal cells (MSCs) have a dynamic secretome that plays a critical role in tissue repair and regeneration. However, studying the MSC secretome in mixed-culture disease models remains challenging. This study aimed to develop a mutant methionyl-tRNA synthetase-based toolkit (MetRS We used CRISPR/Cas9 homology-directed repair to stably integrate MetRS Our results showed successful integration of MetRS The MetRS
Sections du résumé
BACKGROUND
Mesenchymal stromal cells (MSCs) have a dynamic secretome that plays a critical role in tissue repair and regeneration. However, studying the MSC secretome in mixed-culture disease models remains challenging. This study aimed to develop a mutant methionyl-tRNA synthetase-based toolkit (MetRS
METHODS
We used CRISPR/Cas9 homology-directed repair to stably integrate MetRS
RESULTS
Our results showed successful integration of MetRS
CONCLUSIONS
The MetRS
Identifiants
pubmed: 37798772
doi: 10.1186/s13287-023-03515-0
pii: 10.1186/s13287-023-03515-0
pmc: PMC10557244
doi:
Substances chimiques
Methionine-tRNA Ligase
EC 6.1.1.10
Lipopolysaccharides
0
Amino Acids
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
289Subventions
Organisme : NIA NIH HHS
ID : F99 AG068509
Pays : United States
Commentaires et corrections
Type : UpdateOf
Informations de copyright
© 2023. BioMed Central Ltd., part of Springer Nature.
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