Antagonistic RALF peptides control an intergeneric hybridization barrier on Brassicaceae stigmas.
Brassicaceae
CrRLK1L receptors
RALF
interspecific/intergeneric hybridization barrier
pollen mentor effect
pollen-pistil interaction
reproductive isolation
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
26 10 2023
26 10 2023
Historique:
received:
12
11
2022
revised:
24
05
2023
accepted:
06
09
2023
pmc-release:
26
10
2024
medline:
30
10
2023
pubmed:
9
10
2023
entrez:
8
10
2023
Statut:
ppublish
Résumé
Pollen-pistil interactions establish interspecific/intergeneric pre-zygotic hybridization barriers in plants. The rejection of undesired pollen at the stigma is crucial to avoid outcrossing but can be overcome with the support of mentor pollen. The mechanisms underlying this hybridization barrier are largely unknown. Here, in Arabidopsis, we demonstrate that receptor-like kinases FERONIA/CURVY1/ANJEA/HERCULES RECEPTOR KINASE 1 and cell wall proteins LRX3/4/5 interact on papilla cell surfaces with autocrine stigmatic RALF1/22/23/33 peptide ligands (sRALFs) to establish a lock that blocks the penetration of undesired pollen tubes. Compatible pollen-derived RALF10/11/12/13/25/26/30 peptides (pRALFs) act as a key, outcompeting sRALFs and enabling pollen tube penetration. By treating Arabidopsis stigmas with synthetic pRALFs, we unlock the barrier, facilitating pollen tube penetration from distantly related Brassicaceae species and resulting in interspecific/intergeneric hybrid embryo formation. Therefore, we uncover a "lock-and-key" system governing the hybridization breadth of interspecific/intergeneric crosses in Brassicaceae. Manipulating this system holds promise for facilitating broad hybridization in crops.
Identifiants
pubmed: 37806310
pii: S0092-8674(23)00981-9
doi: 10.1016/j.cell.2023.09.003
pmc: PMC10615786
mid: NIHMS1931855
pii:
doi:
Substances chimiques
Arabidopsis Proteins
0
Peptide Hormones
0
Peptides
0
RALF1 protein, Arabidopsis
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4773-4787.e12Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM131827
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests A patent application (no. 202311098597.3) for the pRALFs and their potential applications in achieving distant hybridization in plants has been submitted to the State Intellectual Property Office of China.
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