Progenitor division and cell autonomous neurosecretion are required for rod photoreceptor sublaminar positioning.
migration
neurosecretion
passive displacement
retina
rod photoreceptor
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
17 10 2023
17 10 2023
Historique:
pmc-release:
09
04
2024
medline:
1
11
2023
pubmed:
9
10
2023
entrez:
9
10
2023
Statut:
ppublish
Résumé
Migration is essential for the laminar stratification and connectivity of neurons in the central nervous system. In the retina, photoreceptors (PRs) migrate to positions according to birthdate, with early-born cells localizing to the basal-most side of the outer nuclear layer. It was proposed that apical progenitor mitoses physically drive these basal translocations non-cell autonomously, but direct evidence is lacking, and whether other mechanisms participate is unknown. Here, combining loss- or gain-of-function assays to manipulate cell cycle regulators (Sonic hedgehog, Cdkn1a/p21) with an in vivo lentiviral labelling strategy, we demonstrate that progenitor division is one of two forces driving basal translocation of rod soma. Indeed, replacing Shh activity rescues abnormal rod translocation in retinal explants. Unexpectedly, we show that rod differentiation also promotes rod soma translocation. While outer segment function or formation is dispensable,
Identifiants
pubmed: 37812728
doi: 10.1073/pnas.2308204120
pmc: PMC10589646
doi:
Substances chimiques
Hedgehog Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e2308204120Références
Mol Biosyst. 2010 Jan;6(1):44-54
pubmed: 20024066
Hum Mol Genet. 2005 Oct 15;14(20):3035-46
pubmed: 16155113
Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):16-22
pubmed: 14603031
Nat Neurosci. 2002 Sep;5(9):831-2
pubmed: 12195432
Elife. 2020 May 07;9:
pubmed: 32378514
J Cell Biol. 2005 Dec 19;171(6):991-9
pubmed: 16365165
Development. 2001 Dec;128(24):5099-108
pubmed: 11748145
J Cell Biol. 2016 Oct 24;215(2):259-275
pubmed: 27810916
Cell Rep. 2021 Feb 2;34(5):108698
pubmed: 33535040
EMBO J. 2021 Nov 15;40(22):e107264
pubmed: 34494680
Cell Rep. 2018 May 22;23(8):2416-2428
pubmed: 29791852
J Clin Invest. 2014 Feb;124(2):631-43
pubmed: 24382353
Elife. 2019 Sep 17;8:
pubmed: 31526478
Cell Rep. 2019 Mar 26;26(13):3537-3550.e4
pubmed: 30917310
Proc Natl Acad Sci U S A. 2013 Sep 10;110(37):15109-14
pubmed: 23980162
Nat Genet. 2001 Dec;29(4):447-52
pubmed: 11694879
Nat Genet. 1999 Dec;23(4):466-70
pubmed: 10581037
Stem Cells. 2015 Dec;33(12):3504-18
pubmed: 26235913
Cell. 1997 Nov 14;91(4):531-41
pubmed: 9390562
J Cell Biol. 2013 Sep 2;202(5):725-34
pubmed: 23999166
Proc Natl Acad Sci U S A. 2018 Aug 21;115(34):E7987-E7996
pubmed: 30072429
PLoS One. 2012;7(10):e47180
pubmed: 23071752
Cell Mol Life Sci. 2020 Apr;77(7):1251-1266
pubmed: 31586239
J Cell Sci. 2016 Feb 15;129(4):729-42
pubmed: 26743081
Cell Rep. 2021 Aug 3;36(5):109461
pubmed: 34348137
Elife. 2023 Jan 04;12:
pubmed: 36598133
Neuron. 2008 Oct 9;60(1):84-96
pubmed: 18940590
Sci Rep. 2022 Feb 21;12(1):2897
pubmed: 35190581
Prog Retin Eye Res. 2014 Sep;42:44-84
pubmed: 24984227
Neuron. 2016 Feb 3;89(3):536-49
pubmed: 26844833
Nat Neurosci. 2002 Jan;5(1):19-26
pubmed: 11753414
Neuron. 2018 Apr 4;98(1):109-126.e8
pubmed: 29576390
Front Neurosci. 2018 Jan 09;11:742
pubmed: 29375289
Anat Rec. 1985 Jun;212(2):199-205
pubmed: 3842042
Development. 2004 Mar;131(6):1343-51
pubmed: 14973293
BMC Neurosci. 2005 Jan 27;6:5
pubmed: 15676071
Dev Biol. 2021 Aug;476:218-239
pubmed: 33848537
Nature. 1992 Oct 29;359(6398):832-5
pubmed: 1331807
iScience. 2021 Jul 26;24(8):102905
pubmed: 34430805
Mol Cell Neurosci. 2006 Jul;32(3):274-82
pubmed: 16815712
J Comp Neurol. 1997 Nov 10;388(1):47-63
pubmed: 9364238
Elife. 2022 Dec 02;11:
pubmed: 36459481
Nature. 2023 Aug;620(7974):615-624
pubmed: 37558872
Development. 2015 Aug 1;142(15):2665-77
pubmed: 26116662
Development. 2005 Nov;132(22):5103-13
pubmed: 16236765
Hum Mol Genet. 2011 Mar 15;20(6):1061-73
pubmed: 21177258
Genes Dev. 2001 Dec 1;15(23):3059-87
pubmed: 11731473
Development. 2019 Jan 9;146(1):
pubmed: 30626593
Neural Dev. 2006 Oct 13;1:2
pubmed: 17147778
Elife. 2017 Nov 06;6:
pubmed: 29106373
Channels (Austin). 2013 Nov-Dec;7(6):514-23
pubmed: 24064553
Neuron. 2015 Sep 23;87(6):1248-1260
pubmed: 26402607
Nat Rev Neurosci. 2020 Jan;21(1):5-20
pubmed: 31780820