Laser nanostructured gold biosensor for proto-oncogene detection.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 10 2023
11 10 2023
Historique:
received:
06
07
2023
accepted:
07
10
2023
medline:
1
11
2023
pubmed:
12
10
2023
entrez:
11
10
2023
Statut:
epublish
Résumé
The advancement of biosensor research has been a primary driving force in the continuing progress of modern medical science. While traditional nanofabrication methods have long been the foundation of biosensor research, recent years have seen a shift in the field of nanofabrication towards laser-based techniques. Here we report a gold-based biosensor, with a limit of detection (LoD) 3.18 µM, developed using environmentally friendly Laser Ablation Synthesis in Liquid (LASiS) and Confined Atmospheric Pulsed-laser (CAP) deposition techniques for the first time. The sensors were able detect a DNA fragment corresponding to the longest unpaired sequence of the c-Myc gene, indicating their potential for detecting such fragments in the ctDNA signature of various cancers. The LoD of the developed novel biosensor highlights its reliability and sensitivity as an analytical platform. The reproducibility of the sensor was examined via the production and testing of 200 sensors with the same fabrication methodology. This work offers a scalable, and green approach to fabricating viable biosensors capable of detecting clinically relevant oncogenic targets.
Identifiants
pubmed: 37821490
doi: 10.1038/s41598-023-44372-4
pii: 10.1038/s41598-023-44372-4
pmc: PMC10567688
doi:
Substances chimiques
Gold
7440-57-5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17196Informations de copyright
© 2023. Springer Nature Limited.
Références
Genes (Basel). 2019 Mar 22;10(3):
pubmed: 30909496
Biomark Med. 2019 Aug;13(11):917-929
pubmed: 31144531
Small. 2021 Oct;17(41):e2102694
pubmed: 34510709
Mol Cell Biol. 2004 May;24(10):4294-308
pubmed: 15121849
Signal Transduct Target Ther. 2020 Jul 10;5(1):124
pubmed: 32651356
Int J Mol Sci. 2020 Oct 18;21(20):
pubmed: 33081056
Environ Sci Pollut Res Int. 2022 Jan;29(2):1677-1695
pubmed: 34689274
Nucleic Acids Res. 2003 Jul 1;31(13):3406-15
pubmed: 12824337
Cold Spring Harb Perspect Med. 2014 Jun 02;4(6):
pubmed: 24890832
Nanomaterials (Basel). 2022 Mar 21;12(6):
pubmed: 35335837
Mol Oncol. 2021 Jan;15(1):67-79
pubmed: 32881280
Biosens Bioelectron. 2009 Mar 15;24(7):2071-6
pubmed: 19101134
Talanta. 2001 Jul 6;54(6):1095-103
pubmed: 18968331
Sensors (Basel). 2021 Feb 05;21(4):
pubmed: 33562639
Biosens Bioelectron. 2022 Jul 15;208:114217
pubmed: 35367702
Mol Cancer. 2021 Jan 4;20(1):3
pubmed: 33397405
Anal Bioanal Chem. 2005 Mar;381(5):1004-11
pubmed: 15702308
Nano Lett. 2021 Mar 24;21(6):2422-2428
pubmed: 33720738
Nat Commun. 2021 Jun 25;12(1):3979
pubmed: 34172742
J Hematol Oncol. 2021 Aug 9;14(1):121
pubmed: 34372899
Clin Cancer Res. 2012 Oct 15;18(20):5546-53
pubmed: 23071356
Sci Rep. 2019 Feb 7;9(1):1611
pubmed: 30733532