Biochemical and gene expression alterations due to individual exposure of atrazine, dichlorvos, and imidacloprid and their combination in zebrafish.
Animals
Atrazine
/ metabolism
Antioxidants
/ metabolism
Zebrafish
/ metabolism
Dichlorvos
/ toxicity
Acetylcholinesterase
/ metabolism
Water Pollutants, Chemical
/ metabolism
Oxidative Stress
Catalase
/ metabolism
Glutathione
/ metabolism
Gene Expression
Superoxide Dismutase
/ metabolism
Pesticides
/ metabolism
RNA, Messenger
/ metabolism
Aquatic ecotoxicology
Combined toxicity
Oxidative stress
Pesticide pollution
Synergistic effects
mRNA expression
Journal
Environmental science and pollution research international
ISSN: 1614-7499
Titre abrégé: Environ Sci Pollut Res Int
Pays: Germany
ID NLM: 9441769
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
04
03
2023
accepted:
25
09
2023
medline:
7
12
2023
pubmed:
12
10
2023
entrez:
11
10
2023
Statut:
ppublish
Résumé
In environmental toxicology, combined toxicity has emerged as an important concern. Atrazine (ATZ), dichlorvos (DIC), and imidacloprid (IMD) are the major pesticides, extensively used to control insect, flies, mosquitoes, and weed. Here, we investigate whether the exposure to three different types of pesticides individually and in combination for 24 h alters antioxidant enzyme responses in zebrafish (Danio rerio). Oxidative stress parameters (biochemical and mRNA expression), acetylcholinesterase (AChE) activity, and Metallothionein-II (MT-II) mRNA expression levels were measured. Present work includes toxicological assessment of individual and combined (CMD) exposure of ATZ (185.4 µM), DIC (181 µM), IMD (97.8 µ), and CMD (ATZ 92.7 µM + DIC 90.5 µM + IMD 48.9 µM), in the liver, kidney, and brain of adult zebrafish. Lipid peroxidation (LPO), glutathione (GSH) content, AChE, superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity along with mRNA expression of SOD, CAT, GPx, and MT-II were evaluated. Briefly, LPO, GSH content, the activity of AChE, and all antioxidant enzymes enhanced significantly in individual exposure, which was further altered in the CMD group. The mRNA expression of SOD, CAT, GPx, and MT-II in the liver and kidney showed significant down-regulation in all exposed groups. In the brain, significant upregulation in mRNA expression of SOD, CAT, GPx, and MT-II was observed in DIC and IMD groups, while ATZ and CMD showed significant downregulation except for GPx. Findings postulate that the CMD group exhibits synergistic toxic manifestation. The present study provides the baseline data on the combined toxic effects of pesticides and suggests regulating the use of pesticides.
Identifiants
pubmed: 37821735
doi: 10.1007/s11356-023-30160-0
pii: 10.1007/s11356-023-30160-0
doi:
Substances chimiques
Atrazine
QJA9M5H4IM
Antioxidants
0
imidacloprid
3BN7M937V8
Dichlorvos
7U370BPS14
Acetylcholinesterase
EC 3.1.1.7
Water Pollutants, Chemical
0
Catalase
EC 1.11.1.6
Glutathione
GAN16C9B8O
Superoxide Dismutase
EC 1.15.1.1
Pesticides
0
RNA, Messenger
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
118291-118303Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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