Agrobacterium tumefaciens-mediated transformation of the white-rot fungus Dichomitus squalens.
Agrobacterium tumefaciens-mediated transformation
Dichomitus squalens
DsRed-Express2
EHA105
nptII
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
24
08
2023
revised:
08
10
2023
accepted:
09
10
2023
medline:
10
11
2023
pubmed:
13
10
2023
entrez:
12
10
2023
Statut:
ppublish
Résumé
Dichomitus squalens is an efficient white-rot fungus that generates a wide range of extracellular enzymes to degrade lignocellulose in nature. Although a protoplast-mediated transformation method for D. squalens has been developed, the transformation efficiency remains low. Here, we established a highly efficient Agrobacterium tumefaciens-mediated transformation (ATMT) procedure for D. squalens by transferring a binary vector harboring the neomycin phosphotransferase II (nptII) resistance gene fused with DsRed-Express2, under the control of the native glyceraldehyde-3-phosphate dehydrogenase (GPD) gene promoter. Key factors affecting the efficiency of transformation were tested. A. tumefaciens EHA105 strain with a cell density of 0.4 OD
Identifiants
pubmed: 37827437
pii: S0167-7012(23)00176-8
doi: 10.1016/j.mimet.2023.106842
pii:
doi:
Substances chimiques
Kanamycin Kinase
EC 2.7.1.95
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106842Informations de copyright
Copyright © 2023 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest None.