Experimental phasing opportunities for macromolecular crystallography at very long wavelengths.
Journal
Communications chemistry
ISSN: 2399-3669
Titre abrégé: Commun Chem
Pays: England
ID NLM: 101725670
Informations de publication
Date de publication:
12 Oct 2023
12 Oct 2023
Historique:
received:
27
04
2023
accepted:
26
09
2023
medline:
13
10
2023
pubmed:
13
10
2023
entrez:
12
10
2023
Statut:
epublish
Résumé
Despite recent advances in cryo-electron microscopy and artificial intelligence-based model predictions, a significant fraction of structure determinations by macromolecular crystallography still requires experimental phasing, usually by means of single-wavelength anomalous diffraction (SAD) techniques. Most synchrotron beamlines provide highly brilliant beams of X-rays of between 0.7 and 2 Å wavelength. Use of longer wavelengths to access the absorption edges of biologically important lighter atoms such as calcium, potassium, chlorine, sulfur and phosphorus for native-SAD phasing is attractive but technically highly challenging. The long-wavelength beamline I23 at Diamond Light Source overcomes these limitations and extends the accessible wavelength range to λ = 5.9 Å. Here we report 22 macromolecular structures solved in this extended wavelength range, using anomalous scattering from a range of elements which demonstrate the routine feasibility of lighter atom phasing. We suggest that, in light of its advantages, long-wavelength crystallography is a compelling option for experimental phasing.
Identifiants
pubmed: 37828292
doi: 10.1038/s42004-023-01014-0
pii: 10.1038/s42004-023-01014-0
pmc: PMC10570326
doi:
Types de publication
Journal Article
Langues
eng
Pagination
219Subventions
Organisme : Medical Research Council
ID : MR/S007555/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/K018779/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/V001329/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/L009528/1
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Informations de copyright
© 2023. Springer Nature Limited.
Références
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
pubmed: 21460454
J Mol Biol. 2021 Apr 30;433(9):166893
pubmed: 33639211
Nat Commun. 2019 Sep 2;10(1):3957
pubmed: 31477712
Acta Crystallogr D Struct Biol. 2016 Mar;72(Pt 3):346-58
pubmed: 26960122
Biochem J. 2019 Dec 12;476(23):3649-3660
pubmed: 31802112
Q Rev Biophys. 2014 Feb;47(1):49-93
pubmed: 24726017
Acta Crystallogr D Struct Biol. 2019 Oct 1;75(Pt 10):861-877
pubmed: 31588918
Acta Crystallogr D Struct Biol. 2016 Nov 1;72(Pt 11):1174-1180
pubmed: 27841750
Acta Crystallogr D Struct Biol. 2016 Mar;72(Pt 3):430-9
pubmed: 26960130
Nat Commun. 2017 Apr 03;8:14861
pubmed: 28367989
Sci Rep. 2018 Jan 8;8(1):41
pubmed: 29311713
Acta Crystallogr F Struct Biol Commun. 2021 Jul 1;77(Pt 7):202-207
pubmed: 34196610
Nature. 1981 Mar 12;290(5802):107-113
pubmed: 28769131
Nucleic Acids Res. 2019 Jan 8;47(D1):D520-D528
pubmed: 30357364
IUCrJ. 2022 Oct 21;9(Pt 6):768-777
pubmed: 36381147
Nature. 2022 Aug;608(7921):15-16
pubmed: 35902752
J Biol Chem. 2018 Apr 6;293(14):5064-5078
pubmed: 29449376
Nucleic Acids Res. 2020 Sep 25;48(17):9886-9898
pubmed: 32453431
Nat Commun. 2013;4:2777
pubmed: 24231803
Mol Membr Biol. 2008 Dec;25(8):677-82
pubmed: 19023693
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):479-85
pubmed: 20383001
IUCrJ. 2020 Sep 09;7(Pt 6):965-975
pubmed: 33209311
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2009 May 1;65(Pt 5):463-6
pubmed: 19407377
Science. 2021 Aug 20;373(6557):871-876
pubmed: 34282049
J Mol Biol. 2007 Oct 19;373(2):382-400
pubmed: 17850818
IUCrJ. 2015 May 14;2(Pt 4):431-40
pubmed: 26175902
EMBO J. 2004 Sep 15;23(18):3589-98
pubmed: 15343268
Acta Crystallogr D Biol Crystallogr. 2013 Nov;69(Pt 11):2167-73
pubmed: 24189227
Proc Natl Acad Sci U S A. 2013 Mar 5;110(10):3806-11
pubmed: 23431194
Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9145-50
pubmed: 24920594
J Virol. 2018 Dec 10;93(1):
pubmed: 30305351
J Appl Crystallogr. 2011 Dec 1;44(Pt 6):1285-1287
pubmed: 22477786
J Vis Exp. 2021 Apr 23;(170):
pubmed: 33970149
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
Nat Methods. 2015 Feb;12(2):131-3
pubmed: 25506719
Biochemistry. 1997 May 6;36(18):5578-88
pubmed: 9154942
Science. 2012 May 25;336(6084):1033-7
pubmed: 22628655
IUCrJ. 2020 Oct 14;7(Pt 6):1092-1101
pubmed: 33209320
Nat Commun. 2018 Oct 31;9(1):4540
pubmed: 30382100
Protein Sci. 2022 Jan;31(1):232-250
pubmed: 34747533
Acta Crystallogr D Biol Crystallogr. 2008 Nov;64(Pt 11):1179-82
pubmed: 19020357
Acta Crystallogr D Biol Crystallogr. 2010 Apr;66(Pt 4):486-501
pubmed: 20383002
Acta Crystallogr D Biol Crystallogr. 2014 Aug;70(Pt 8):2054-68
pubmed: 25084326
Acta Crystallogr D Biol Crystallogr. 2006 Sep;62(Pt 9):1002-11
pubmed: 16929101
Science. 2022 Jun 10;376(6598):eabm9326
pubmed: 35679401
EMBO J. 2017 Oct 16;36(20):3062-3079
pubmed: 28864543
Proc Natl Acad Sci U S A. 2018 May 8;115(19):E4350-E4357
pubmed: 29666242
IUCrJ. 2019 Apr 01;6(Pt 3):373-386
pubmed: 31098019
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32
pubmed: 20124692
Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1204-14
pubmed: 23793146
BMC Struct Biol. 2005 Aug 10;5:13
pubmed: 16092953
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
Methods Enzymol. 1985;115:90-112
pubmed: 4079800
Acta Crystallogr F Struct Biol Commun. 2022 Feb 1;78(Pt 2):88-95
pubmed: 35102898
J Mol Biol. 2000 Dec 1;304(3):397-410
pubmed: 11090282
Science. 2014 Mar 28;343(6178):1443-4
pubmed: 24675944
Acta Crystallogr D Struct Biol. 2018 Feb 1;74(Pt 2):117-124
pubmed: 29533237