Ancient DNA is preserved in fish fossils from tropical lake sediments.
adaptive radiation
cichlid fish
conservation
diversification
evolution
palaeogenetics
Journal
Molecular ecology
ISSN: 1365-294X
Titre abrégé: Mol Ecol
Pays: England
ID NLM: 9214478
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
revised:
30
08
2023
received:
18
04
2023
accepted:
25
09
2023
medline:
7
11
2023
pubmed:
13
10
2023
entrez:
13
10
2023
Statut:
ppublish
Résumé
Tropical freshwater lakes are well known for their high biodiversity, and particularly the East African Great Lakes are renowned for their adaptive radiation of cichlid fishes. While comparative phylogenetic analyses of extant species flocks have revealed patterns and processes of their diversification, little is known about evolutionary trajectories within lineages, the impacts of environmental drivers, or the scope and nature of now-extinct diversity. Time-structured palaeodata from geologically young fossil records, such as fossil counts and particularly ancient DNA (aDNA) data, would help fill this large knowledge gap. High ambient temperatures can be detrimental to the preservation of DNA, but refined methodology now allows data generation even from very poorly preserved samples. Here, we show for the first time that fish fossils from tropical lake sediments yield endogenous aDNA. Despite generally low endogenous content and high sample dropout, the application of high-throughput sequencing and, in some cases, sequence capture allowed taxonomic assignment and phylogenetic placement of 17% of analysed fish fossils to family or tribe level, including remains which are up to 2700 years old or weigh less than 1 mg. The relationship between aDNA degradation and the thermal age of samples is similar to that described for terrestrial samples from cold environments when adjusted for elevated temperature. Success rates and aDNA preservation differed between the investigated lakes Chala, Kivu and Victoria, possibly caused by differences in bottom water oxygenation. Our study demonstrates that the sediment records of tropical lakes can preserve genetic information on rapidly diversifying fish taxa over time scales of millennia.
Substances chimiques
DNA, Ancient
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5913-5931Subventions
Organisme : Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
ID : CRSII5_183566
Organisme : FP7 Ideas: European Research Council
ID : 324249
Organisme : Strategy pool of the Faculty of Natural Sciences of the University of Bern
Organisme : MacArthur Foundation
ID : 11-97251-000-INP
Informations de copyright
© 2023 The Authors. Molecular Ecology published by John Wiley & Sons Ltd.
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