Cytoplasmic division cycles without the nucleus and mitotic CDK/cyclin complexes.
Cdk
Drosophila embryo
autonomous clocks
cell cycle
centrosome
cyclin
cytokinesis
epithelial homeostasis
microtubules
mitosis
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
12 10 2023
12 10 2023
Historique:
received:
05
06
2022
revised:
11
05
2023
accepted:
12
09
2023
medline:
2
11
2023
pubmed:
14
10
2023
entrez:
13
10
2023
Statut:
ppublish
Résumé
Cytoplasmic divisions are thought to rely on nuclear divisions and mitotic signals. We demonstrate in Drosophila embryos that cytoplasm can divide repeatedly without nuclei and mitotic CDK/cyclin complexes. Cdk1 normally slows an otherwise faster cytoplasmic division cycle, coupling it with nuclear divisions, and when uncoupled, cytoplasm starts dividing before mitosis. In developing embryos where CDK/cyclin activity can license mitotic microtubule (MT) organizers like the spindle, cytoplasmic divisions can occur without the centrosome, a principal organizer of interphase MTs. However, centrosomes become essential in the absence of CDK/cyclin activity, implying that the cytoplasm can employ either the centrosome-based interphase or CDK/cyclin-dependent mitotic MTs to facilitate its divisions. Finally, we present evidence that autonomous cytoplasmic divisions occur during unperturbed fly embryogenesis and that they may help extrude mitotically stalled nuclei during blastoderm formation. We postulate that cytoplasmic divisions occur in cycles governed by a yet-to-be-uncovered clock mechanism autonomous from CDK/cyclin complexes.
Identifiants
pubmed: 37832525
pii: S0092-8674(23)01031-0
doi: 10.1016/j.cell.2023.09.010
pmc: PMC10659773
mid: NIHMS1937362
pii:
doi:
Substances chimiques
Cyclins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4694-4709.e16Subventions
Organisme : NIGMS NIH HHS
ID : DP2 GM154328
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM136420
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM149436
Pays : United States
Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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