Chlorogenic acid induces apoptosis and cell-cycle arrest in colorectal cancer cells.
Humans
Chlorogenic Acid
/ pharmacology
Caspase 3
/ genetics
Reactive Oxygen Species
/ metabolism
NF-kappa B
/ metabolism
HEK293 Cells
Tumor Suppressor Protein p53
/ genetics
Cell Cycle Checkpoints
Apoptosis
Proto-Oncogene Proteins c-bcl-2
/ metabolism
Colonic Neoplasms
/ metabolism
Colorectal Neoplasms
/ drug therapy
Cell Line, Tumor
Cell Proliferation
Caspase 9
Cell cycle
HEK-293
HT-29 cells
P21
ROS
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
14
05
2023
accepted:
26
09
2023
medline:
27
11
2023
pubmed:
17
10
2023
entrez:
17
10
2023
Statut:
ppublish
Résumé
Apoptotic agents from natural products like phenolic compounds can be used effectively in the treatment of cancer. Chlorogenic acid (CGA) is one of the phenolic compounds in medicinal plants with anti-cancer properties. In this research, we aimed to explore the anti-cancer mode of action of CGA on colorectal cancer (CRC) cells in vitro conditions. HT-29 and HEK-293 cells were cultured after MTT assay for 24 h with CGA 100 µM, and without CGA. Then, flow cytometry assays and the expression of apoptosis-related genes including caspase 3 and 9, Bcl-2 and Bax, and cell cycle-related genes including P21, P53 and NF-κB at mRNA and protein levels were examined. Finally, we measured the amount of intracellular reactive oxygen species (ROS). The cell viability of all two-cell lines decreased in a dose-dependent manner. Moreover, CGA induces cell cycle arrest in HT-29 cells by increasing the expression of P21 and P53. It also induces apoptosis in HT-29 cells by mitigating Bcl-2 and NF-κB expression and elevating caspase 3 and 9 expression and ROS levels. Considering the cytotoxicity and cell cycle arrest and induction of apoptosis in the colon cancer cell line by CGA, it can be concluded that CGA is a suitable option for the treatment of colon cancer.
Sections du résumé
BACKGROUND
BACKGROUND
Apoptotic agents from natural products like phenolic compounds can be used effectively in the treatment of cancer. Chlorogenic acid (CGA) is one of the phenolic compounds in medicinal plants with anti-cancer properties. In this research, we aimed to explore the anti-cancer mode of action of CGA on colorectal cancer (CRC) cells in vitro conditions.
METHODS
METHODS
HT-29 and HEK-293 cells were cultured after MTT assay for 24 h with CGA 100 µM, and without CGA. Then, flow cytometry assays and the expression of apoptosis-related genes including caspase 3 and 9, Bcl-2 and Bax, and cell cycle-related genes including P21, P53 and NF-κB at mRNA and protein levels were examined. Finally, we measured the amount of intracellular reactive oxygen species (ROS).
RESULTS
RESULTS
The cell viability of all two-cell lines decreased in a dose-dependent manner. Moreover, CGA induces cell cycle arrest in HT-29 cells by increasing the expression of P21 and P53. It also induces apoptosis in HT-29 cells by mitigating Bcl-2 and NF-κB expression and elevating caspase 3 and 9 expression and ROS levels.
CONCLUSIONS
CONCLUSIONS
Considering the cytotoxicity and cell cycle arrest and induction of apoptosis in the colon cancer cell line by CGA, it can be concluded that CGA is a suitable option for the treatment of colon cancer.
Identifiants
pubmed: 37847443
doi: 10.1007/s11033-023-08854-y
pii: 10.1007/s11033-023-08854-y
doi:
Substances chimiques
Chlorogenic Acid
318ADP12RI
Caspase 3
EC 3.4.22.-
Reactive Oxygen Species
0
NF-kappa B
0
Tumor Suppressor Protein p53
0
Proto-Oncogene Proteins c-bcl-2
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9845-9857Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
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