Production of extracellular agarase from Priestia megaterium AT7 and evaluation on marine algae hydrolysis.
Agar
Algae hydrolysis
Extracellular agarase
Molecular identification
Priestia megaterium
Journal
Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761
Informations de publication
Date de publication:
Jan 2024
Jan 2024
Historique:
received:
09
12
2022
revised:
03
10
2023
accepted:
04
10
2023
medline:
20
11
2023
pubmed:
20
10
2023
entrez:
19
10
2023
Statut:
ppublish
Résumé
Agar is a common component biosynthesized from various marine algae species that is widely applied in various fields including food and pharmaceutical industries. However, the structural composition of agar is highly resisted against chemical and biological hydrolysis. Therefore, tremendous research is exploring various pretreatment strategies to break down the intrinsic chemical structural of agar linkage (i.e. neutral agarose and highly sulfated agaropectin) prior for its industrial potential usage. In this research work, a novel agar degrading bacterium was screened and isolated from agriculture soils. Molecular identification using nucleotide sequence of 16 s rRNA region comparison has indicated that the isolate belonged to Priestia genus, and was identified as Priestia megaterium AT7. The maximum enzyme activity was 52.85 ± 1.76 U/mL after 96 h of culture with 5% inoculum size and agitation speed of 180 rpm. Results indicated that the optimal condition for the production of agarose was achieved at pH 7 at 50 °C. The effects of metal ions (e.g. Ca
Identifiants
pubmed: 37857079
pii: S0141-0229(23)00147-3
doi: 10.1016/j.enzmictec.2023.110339
pii:
doi:
Substances chimiques
agarase
EC 3.2.1.81
Agar
9002-18-0
Sepharose
9012-36-6
Sugars
0
Glycoside Hydrolases
EC 3.2.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110339Informations de copyright
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