Blocking of ebolavirus spread through intercellular connections by an MPER-specific antibody depends on BST2/tetherin.

Humans Antibodies, Monoclonal Antibodies, Viral Antigens, CD Bone Marrow Stromal Antigen 2 / immunology Ebolavirus / immunology GPI-Linked Proteins Hemorrhagic Fever, Ebola / virology
BST2 CP: Immunology CP: Microbiology Ebola virus MPER TIM-1 cell-to-cell transmission intercellular connections monoclonal antibodies tetherin virus egress

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
31 10 2023
Historique:
received: 05 12 2022
revised: 10 08 2023
accepted: 27 09 2023
medline: 6 11 2023
pubmed: 20 10 2023
entrez: 20 10 2023
Statut: ppublish

Résumé

Ebola virus (EBOV) and Bundibugyo virus (BDBV) belong to the family Filoviridae and cause a severe disease in humans. We previously isolated a large panel of monoclonal antibodies from B cells of human survivors from the 2007 Uganda BDBV outbreak, 16 survivors from the 2014 EBOV outbreak in the Democratic Republic of the Congo, and one survivor from the West African 2013-2016 EBOV epidemic. Here, we demonstrate that EBOV and BDBV are capable of spreading to neighboring cells through intercellular connections in a process that depends upon actin and T cell immunoglobulin and mucin 1 protein. We quantify spread through intercellular connections by immunofluorescence microscopy and flow cytometry. One of the antibodies, BDBV223, specific to the membrane-proximal external region, induces virus accumulation at the plasma membrane. The inhibiting activity of BDBV223 depends on BST2/tetherin.

Identifiants

DOI: 10.1016/j.celrep.2023.113254 PMID: 37858466 PMC: PMC10664807
pubmed: 37858466
pii: S2211-1247(23)01266-4
doi: 10.1016/j.celrep.2023.113254
pmc: PMC10664807
mid: NIHMS1941925
pii:
doi:

Substances chimiques

Antibodies, Monoclonal 0
Antibodies, Viral 0
Antigens, CD 0
Bone Marrow Stromal Antigen 2 0
BST2 protein, human 0
GPI-Linked Proteins 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

113254

Subventions

Organisme : NIAID NIH HHS
ID : U19 AI142785
Pays : United States

Informations de copyright

Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no competing interests.

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Auteurs

Rodrigo I Santos (RI)

Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USA.

Philipp A Ilinykh (PA)

Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USA.

Colette A Pietzsch (CA)

Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USA.

Adam J Ronk (AJ)

Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USA.

Kai Huang (K)

Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USA.

Natalia A Kuzmina (NA)

Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USA.

Fuchun Zhou (F)

Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USA.

James E Crowe (JE)

Vanderbilt Vaccine Center, Vanderbilt University Medical Center, Nashville, TN, USA; Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA; Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN, USA. Electronic address: james.crowe@vumc.org.

Alexander Bukreyev (A)

Department of Pathology, University of Texas Medical Branch, Galveston, TX, USA; Galveston National Laboratory, Galveston, TX, USA; Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, USA. Electronic address: alexander.bukreyev@utmb.edu.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Blocking of ebolavirus spread through...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps monoclonaux › Blocking of ebolavirus spread through...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Globulines › Sérum-globulines › Immunoglobulines › Anticorps › Anticorps antiviraux › Blocking of ebolavirus spread through...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Antigènes de différenciation › Antigènes CD › Blocking of ebolavirus spread through...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Glycoprotéines membranaires › Protéines liées au GPI › Antigène stromal-2 de la moëlle osseuse › Blocking of ebolavirus spread through...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN de polarité négative › Mononegavirales › Filoviridae › Ebolavirus › Blocking of ebolavirus spread through...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Glycoprotéines membranaires › Protéines liées au GPI › Blocking of ebolavirus spread through...
questionsmedicales.fr › Infection › Maladies virales › Infections à virus à ARN › Infections à Mononegavirales › Infections à Filoviridae › Fièvre hémorragique à virus Ebola › Blocking of ebolavirus spread through...