Structural snapshots of Mycobacterium tuberculosis enolase reveal dual mode of 2PG binding and its implication in enzyme catalysis.
Mycobacterium tuberculosis
X-ray crystallography
cryo-electron microscopy
enolase
enzyme mechanisms
gluconeogenesis
Journal
IUCrJ
ISSN: 2052-2525
Titre abrégé: IUCrJ
Pays: England
ID NLM: 101623101
Informations de publication
Date de publication:
01 Nov 2023
01 Nov 2023
Historique:
received:
16
05
2023
accepted:
27
09
2023
medline:
2
11
2023
pubmed:
20
10
2023
entrez:
20
10
2023
Statut:
epublish
Résumé
Enolase, a ubiquitous enzyme, catalyzes the reversible conversion of 2-phosphoglycerate (2PG) to phosphoenolpyruvate (PEP) in the glycolytic pathway of organisms of all three domains of life. The underlying mechanism of the 2PG to PEP conversion has been studied in great detail in previous work, however that of the reverse reaction remains to be explored. Here we present structural snapshots of Mycobacterium tuberculosis (Mtb) enolase in apo, PEP-bound and two 2PG-bound forms as it catalyzes the conversion of PEP to 2PG. The two 2PG-bound complex structures differed in the conformation of the bound product (2PG) viz the widely reported canonical conformation and a novel binding pose, which we refer to here as the alternate conformation. Notably, we observed two major differences compared with the forward reaction: the presence of Mg
Identifiants
pubmed: 37860976
pii: S2052252523008485
doi: 10.1107/S2052252523008485
pmc: PMC10619443
doi:
Substances chimiques
Phosphopyruvate Hydratase
EC 4.2.1.11
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
738-753Informations de copyright
open access.
Références
J Struct Biol. 2016 Jan;193(1):1-12
pubmed: 26592709
Front Cell Dev Biol. 2019 Apr 26;7:61
pubmed: 31106201
Nat Commun. 2015 Aug 10;6:7912
pubmed: 26258286
Expert Opin Drug Discov. 2015 May;10(5):449-61
pubmed: 25835573
Protein Sci. 2018 Jan;27(1):112-128
pubmed: 28836357
J Mol Biol. 2004 Oct 29;343(4):997-1005
pubmed: 15476816
Int J Med Microbiol. 2013 Dec;303(8):452-62
pubmed: 23906818
Curr Opin Struct Biol. 1996 Dec;6(6):736-43
pubmed: 8994873
Biochemistry. 1991 Mar 19;30(11):2817-22
pubmed: 2007120
Front Microbiol. 2018 Oct 26;9:2542
pubmed: 30416491
J Struct Biol. 2007 Jan;157(1):281-7
pubmed: 16963278
J Comput Chem. 2005 Dec;26(16):1701-18
pubmed: 16211538
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Nucleic Acids Res. 2016 Jul 8;44(W1):W344-50
pubmed: 27166375
Acta Crystallogr F Struct Biol Commun. 2022 Apr 1;78(Pt 4):177-184
pubmed: 35400670
Mol Microbiol. 2003 Apr;48(1):77-84
pubmed: 12657046
Biochim Biophys Acta. 2008 Jul-Aug;1784(7-8):986-94
pubmed: 18456007
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):235-42
pubmed: 21460441
Acta Crystallogr D Biol Crystallogr. 2015 Dec 1;71(Pt 12):2457-70
pubmed: 26627653
Biochim Biophys Acta Gen Subj. 2017 Jan;1861(1 Pt A):3355-3364
pubmed: 27569900
PLoS One. 2015 Mar 25;10(3):e0121764
pubmed: 25807546
Nat Methods. 2014 Jan;11(1):63-5
pubmed: 24213166
Front Cell Infect Microbiol. 2014 Jun 03;4:66
pubmed: 24918088
Mol Microbiol. 2001 Jun;40(6):1273-87
pubmed: 11442827
Acta Crystallogr D Biol Crystallogr. 2011 Apr;67(Pt 4):355-67
pubmed: 21460454
Am Rev Tuberc. 1957 Mar;75(3):495-500
pubmed: 13403178
F1000Res. 2014 Sep 09;3:214
pubmed: 25685322
Microb Pathog. 2023 Jan;174:105934
pubmed: 36481292
Biochemistry. 1999 Sep 14;38(37):12138-49
pubmed: 10508418
J Med Microbiol. 2003 Aug;52(Pt 8):615-622
pubmed: 12867553
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Nov 1;67(Pt 11):1451-6
pubmed: 22102255
J Comput Chem. 2004 Oct;25(13):1605-12
pubmed: 15264254
Chem Biol Drug Des. 2019 Apr;93(4):522-538
pubmed: 30468569
Acta Crystallogr D Biol Crystallogr. 2004 Aug;60(Pt 8):1355-63
pubmed: 15272157
RNA Biol. 2009 Sep-Oct;6(4):402-5
pubmed: 19667755
Enzyme Res. 2011;2011:932549
pubmed: 21603223
Methods Enzymol. 1997;276:307-26
pubmed: 27754618
Acta Crystallogr D Biol Crystallogr. 2010 May;66(Pt 5):503-13
pubmed: 20445225
PLoS Comput Biol. 2012;8(10):e1002708
pubmed: 23093919
Microb Pathog. 2017 Nov;112:20-29
pubmed: 28942172
Trends Microbiol. 2005 Jul;13(7):308-13
pubmed: 15936195
PLoS Pathog. 2011 Sep;7(9):e1002251
pubmed: 21980284
Protein Sci. 2018 Jan;27(1):135-145
pubmed: 28884485
Protein Cell. 2012 Oct;3(10):769-80
pubmed: 23055041
Biochemistry. 1997 Oct 14;36(41):12526-34
pubmed: 9376357
ACS Omega. 2020 Jul 14;5(29):18356-18366
pubmed: 32743211
Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32
pubmed: 15572765
Biochim Biophys Acta. 1998 Apr 2;1383(2):351-5
pubmed: 9602170
Future Microbiol. 2022 Dec;17:1455-1473
pubmed: 36354018
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21
pubmed: 20124702
Elife. 2018 Nov 09;7:
pubmed: 30412051
Biopolymers. 1983 Dec;22(12):2577-637
pubmed: 6667333
Biochemistry. 2003 Jul 15;42(27):8298-306
pubmed: 12846578
Eur J Biochem. 2003 Aug;270(15):3205-13
pubmed: 12869196
Evolution. 1985 Jul;39(4):783-791
pubmed: 28561359
Proc Natl Acad Sci U S A. 1995 Feb 28;92(5):1530-4
pubmed: 7878014
ACS Catal. 2019 Oct 4;9(10):8985-8990
pubmed: 31853382
J Appl Crystallogr. 2007 Aug 1;40(Pt 4):658-674
pubmed: 19461840
J Chem Theory Comput. 2011 Jul 12;7(7):2284-95
pubmed: 26606496
Comput Appl Biosci. 1992 Jun;8(3):275-82
pubmed: 1633570
J Cell Biochem. 2018 Feb;119(2):2408-2417
pubmed: 28888036
Infect Immun. 2011 Sep;79(9):3476-91
pubmed: 21646455
Insect Biochem Mol Biol. 2009 Nov;39(11):801-13
pubmed: 19786101
J Struct Biol. 2012 Dec;180(3):519-30
pubmed: 23000701