Optimizing the precision of laser speckle contrast imaging.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
20 10 2023
20 10 2023
Historique:
received:
10
07
2023
accepted:
18
10
2023
medline:
31
10
2023
pubmed:
21
10
2023
entrez:
20
10
2023
Statut:
epublish
Résumé
Laser speckle contrast imaging (LSCI) is a rapidly developing technology broadly applied for the full-field characterization of tissue perfusion. Over the recent years, significant advancements have been made in interpreting LSCI measurements and improving the technique's accuracy. On the other hand, the method's precision has yet to be studied in detail, despite being as important as accuracy for many biomedical applications. Here we combine simulation, theory and animal experiments to systematically evaluate and re-analyze the role of key factors defining LSCI precision-speckle-to-pixel size ratio, polarisation, exposure time and camera-related noise. We show that contrary to the established assumptions, smaller speckle size and shorter exposure time can improve the precision, while the camera choice is less critical and does not affect the signal-to-noise ratio significantly.
Identifiants
pubmed: 37864006
doi: 10.1038/s41598-023-45303-z
pii: 10.1038/s41598-023-45303-z
pmc: PMC10589309
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17970Informations de copyright
© 2023. Springer Nature Limited.
Références
Opt Lett. 2014 Feb 1;39(3):678-81
pubmed: 24487897
Biomed Opt Express. 2022 Jul 06;13(8):4118-4133
pubmed: 36032565
IEEE Trans Biomed Eng. 2018 Oct;65(10):2136-2142
pubmed: 28541195
Sci Adv. 2020 Nov 6;6(45):
pubmed: 33158865
J Biomed Opt. 2010 Jan-Feb;15(1):011109
pubmed: 20210435
Opt Lett. 2006 Dec 1;31(23):3465-7
pubmed: 17099751
J Cereb Blood Flow Metab. 2011 Jun;31(6):1452-60
pubmed: 21326267
J Biomed Opt. 2013 Jun;18(6):066018
pubmed: 23807512
Biomed Opt Express. 2016 Feb 08;7(3):798-809
pubmed: 27231589
J Opt Soc Am A Opt Image Sci Vis. 2008 Jan;25(1):231-7
pubmed: 18157231
Sci Rep. 2019 Feb 22;9(1):2542
pubmed: 30796288
Opt Express. 2013 Nov 18;21(23):28902-13
pubmed: 24514404
Sci Rep. 2020 Apr 28;10(1):7177
pubmed: 32346043
PLoS One. 2017 Sep 12;12(9):e0184772
pubmed: 28898284
Biomed Opt Express. 2022 Nov 17;13(12):6533-6549
pubmed: 36589566
Opt Lett. 2022 Dec 15;47(24):6333-6336
pubmed: 36538431
Neurophotonics. 2020 Jan;7(1):015005
pubmed: 32042854
Opt Lett. 2008 Dec 15;33(24):2886-8
pubmed: 19079481
Biomed Opt Express. 2021 May 21;12(6):3571-3583
pubmed: 34221679
Nat Med. 2002 Feb;8(2):136-42
pubmed: 11821897
Opt Express. 2008 Feb 4;16(3):1975-89
pubmed: 18542277
Invest Ophthalmol Vis Sci. 1982 Feb;22(2):255-9
pubmed: 7056639
Opt Lett. 2017 Jun 15;42(12):2299-2301
pubmed: 28614336
Neurophotonics. 2022 Apr;9(2):021908
pubmed: 35265733
J Cereb Blood Flow Metab. 2001 Mar;21(3):195-201
pubmed: 11295873
Nat Protoc. 2009;4(8):1128-44
pubmed: 19617885
J Cereb Blood Flow Metab. 2015 Mar;35(3):367-70
pubmed: 25492111
J Rheumatol. 2015 Jul;42(7):1163-8
pubmed: 26034146
Appl Opt. 2005 Apr 1;44(10):1823-30
pubmed: 15813518
Biomed Opt Express. 2010 Jul 15;1(1):246-259
pubmed: 21258462
Microvasc Res. 2012 May;83(3):376-9
pubmed: 22326551
Biomed Opt Express. 2016 Mar 18;7(4):1375-84
pubmed: 27446662