Unraveling the molecular mechanisms of selenite reduction: transcriptomic analysis of Bacillus reveals the key role of sulfur assimilation.
Bacillus sp.
Selenite reduction
Sulfur assimilation
Transcriptomic sequencing
Journal
Biotechnology letters
ISSN: 1573-6776
Titre abrégé: Biotechnol Lett
Pays: Netherlands
ID NLM: 8008051
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
30
07
2023
accepted:
18
09
2023
revised:
09
09
2023
medline:
10
11
2023
pubmed:
21
10
2023
entrez:
21
10
2023
Statut:
ppublish
Résumé
Selenite biotransformation by microorganisms is an effective detoxification and assimilation process. However, current knowledge of the molecular mechanisms of selenite reduction remains circumscribed. Here, the reduction of Se(IV) by a highly selenite-resistant Bacillus sp. SL (up to 50 mM) was systematically analyzed, and the molecular mechanisms of selenite reduction were investigated. Remarkably, 10 mM selenite was entirely transformed by the strain SL within 20 h, demonstrating a faster conversion rate compared to other microorganisms. Furthermore, glutathione (GSH) and exopolysaccharides (EPS) changes were also monitored during the process. Transcriptomic analysis revealed that the genes of ferredoxin-sulfite oxidoreductase (6.82) and sulfate adenylyltransferase (6.32) were significantly upregulated, indicating that the sulfur assimilation pathway is the primary reducing pathway involved in selenite reduction by strain SL. Moreover, key genes associated with NAD(P)/FAD-dependent oxidoreductases and thioredoxin were significantly upregulated. The reduction of Se(IV) was mediated by multiple pathways in strain SL. To our knowledge, this is the initial report to identify the involvement of sulfur assimilation pathway in selenite reduction for bacillus, which is rare in aerobic bacteria.
Identifiants
pubmed: 37864746
doi: 10.1007/s10529-023-03439-y
pii: 10.1007/s10529-023-03439-y
doi:
Substances chimiques
Selenious Acid
F6A27P4Q4R
Oxidoreductases
EC 1.-
Sodium Selenite
HIW548RQ3W
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1513-1520Subventions
Organisme : Innovative Research Group Project of the National Natural Science Foundation of China
ID : No. 31970107
Organisme : Key Research and Development Program of Liaoning Province
ID : 2018YFC1602306
Organisme : State Key Laboratory of Urban Water Resource and Environment
ID : No. OAK201943
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
Références
Antonioli P, Larnpis S, Chesini I, Vallini G, Rinalducci S, Zolla L, Righetti PG (2007) Stenotrophomonas maltophilia SeITE02, a new bacterial strain suitable for bioremediation of selenite-contaminated environmental matrices. Appl Environ Microbiol 73:6854–6863
doi: 10.1128/AEM.00957-07
pubmed: 17827320
pmcid: 2074961
Che L, Xu WP, Zhan JJ, Zhang L, Liu LF, Zhou H (2019) Complete genome sequence of Bacillus cereus CC-1, a novel marine selenate/selenite reducing bacterium producing metallic selenides nanomaterials. Curr Microbiol 76:78–85
doi: 10.1007/s00284-018-1587-9
pubmed: 30343326
Cory SA, Van Vranken JG, Brignole EJ, Patra S, Winge DR, Drennan CL, Rutter J, Barondeau DP (2017) Structure of human Fe-S assembly subcomplex reveals unexpected cysteine desulfurase architecture and acyl-ACP-ISD11 interactions. Proc Natl Acad Sci USA 114:E5325–E5334
doi: 10.1073/pnas.1702849114
pubmed: 28634302
pmcid: 5502623
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colrimetic method for determination of sugars and related substances. Anal Chem 28:350–356
doi: 10.1021/ac60111a017
Fan S, Yang Y, Sun L, Yu B, Dai C, Qu Y (2022) Different toxicity to liver and gill of zebrafish by selenium nanoparticles derived from bio/chemical methods. Environ Sci Pollut Res Int 29:61512–61521
doi: 10.1007/s11356-022-20295-x
pubmed: 35445301
Ku JWK, Gan YH (2021) New roles for glutathione: modulators of bacterial virulence and pathogenesis. Redox Biol 44:102012
doi: 10.1016/j.redox.2021.102012
pubmed: 34090244
pmcid: 8182430
Lemly AD (2014) An urgent need for an EPA standard for disposal of coal ash. Environ Pollut 191:253–255
doi: 10.1016/j.envpol.2014.04.029
pubmed: 24836055
Lenz M, Kolvenbach B, Gygax B, Moes S, Corvini PFX (2011) shedding light on selenium biomineralization: proteins associated with bionanominerals. Appl Environ Microbiol 77:4676–4680
doi: 10.1128/AEM.01713-10
pubmed: 21602371
pmcid: 3127719
Li BZ, Liu N, Li YQ, Jing WX, Fan JH, Li D, Zhang LY, Zhang XF, Zhang ZM, Wang L (2014) Reduction of selenite to red elemental selenium by rhodopseudomonas palustris strain N. PLoS ONE 9:10
pmcid: 3906163
Li T, Xu H, Zhang Y, Zhang H, Hu X, Sun Y, Gu X, Luo J, Zhou D, Gao B (2022) Treatment technologies for selenium contaminated water: a critical review. Environ Pollut 299:118858
doi: 10.1016/j.envpol.2022.118858
pubmed: 35041898
Martinez FG, Moreno-Martin G, Pescuma M, Madrid-Albarran Y, Mozzi F (2020) biotransformation of selenium by lactic acid bacteria: formation of seleno-nanoparticles and seleno-amino acids. Front Bioeng Biotechnol 8:17
doi: 10.3389/fbioe.2020.00506
Nancharaiah YV, Lens P (2015) Ecology and biotechnology of selenium-respiring bacteria. Microbiol Mol Biol Rev 79:61–80
doi: 10.1128/MMBR.00037-14
pubmed: 25631289
pmcid: 4402961
Nguyen V, Nguyen TH, Ha MG, Kang HY (2019) Kinetics of microbial selenite reduction by novel bacteria isolated from activated sludge. J Environ Manage 236:746–754
doi: 10.1016/j.jenvman.2019.02.012
pubmed: 30772731
Pettine M, Gennari F, Campanella L, Casentini B, Marani D (2012) The reduction of selenium(IV) by hydrogen sulfide in aqueous solutions. Geochim Cosmochim Acta 83:37–47
doi: 10.1016/j.gca.2011.12.024
Wang D, Rensing C, Zheng S (2022) Microbial reduction and resistance to selenium: mechanisms, applications and prospects. J Hazard Mater 421:126684
doi: 10.1016/j.jhazmat.2021.126684
pubmed: 34339989
Wasewar KL, Prasad B, Gulipalli S (2009) Removal of selenium by adsorption onto granular activated carbon (GAC) and powdered activated carbon (PAC). Clean-Soil Air Water 37:872–883
doi: 10.1002/clen.200900188
Winkel LHE, Johnson CA, Lenz M, Grundl T, Leupin OX, Amini M, Charlet L (2012) Environmental selenium research: from microscopic processes to global understanding. Environ Sci Technol 46:571–579
doi: 10.1021/es203434d
pubmed: 22129299
Won S, Ha MG, Nguyen DD, Kang HY (2021) Biological selenite removal and recovery of selenium nanoparticles by haloalkaliphilic bacteria isolated from the Nakdong River. Environ Pollut 280:11
doi: 10.1016/j.envpol.2021.117001
Yang XY, Dai XF, Jin HN, Lin GE, Wang ZH, Song Y, Zhang W, Man CX, Jiang YJ (2021) Physicochemical and transcriptomic responses of Lactobacillus brevis JLD715 to sodium selenite. J Sci Food Agric 101:4332–4341
doi: 10.1002/jsfa.11073
pubmed: 33417239