Acylation of glycerolipids in mycobacteria.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
23 10 2023
23 10 2023
Historique:
received:
02
03
2023
accepted:
12
10
2023
medline:
26
10
2023
pubmed:
24
10
2023
entrez:
23
10
2023
Statut:
epublish
Résumé
We report on the existence of two phosphatidic acid biosynthetic pathways in mycobacteria, a classical one wherein the acylation of the sn-1 position of glycerol-3-phosphate (G3P) precedes that of sn-2 and another wherein acylations proceed in the reverse order. Two unique acyltransferases, PlsM and PlsB2, participate in both pathways and hold the key to the unusual positional distribution of acyl chains typifying mycobacterial glycerolipids wherein unsaturated substituents principally esterify position sn-1 and palmitoyl principally occupies position sn-2. While PlsM selectively transfers a palmitoyl chain to the sn-2 position of G3P and sn-1-lysophosphatidic acid (LPA), PlsB2 preferentially transfers a stearoyl or oleoyl chain to the sn-1 position of G3P and an oleyl chain to sn-2-LPA. PlsM is the first example of an sn-2 G3P acyltransferase outside the plant kingdom and PlsB2 the first example of a 2-acyl-G3P acyltransferase. Both enzymes are unique in their ability to catalyze acyl transfer to both G3P and LPA.
Identifiants
pubmed: 37872138
doi: 10.1038/s41467-023-42478-x
pii: 10.1038/s41467-023-42478-x
pmc: PMC10593935
doi:
Substances chimiques
Acyltransferases
EC 2.3.-
Glycerol-3-Phosphate O-Acyltransferase
EC 2.3.1.15
alpha-glycerophosphoric acid
9NTI6P3O4X
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6694Subventions
Organisme : NIAID NIH HHS
ID : R01 AI064798
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI155674
Pays : United States
Informations de copyright
© 2023. Springer Nature Limited.
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