Challenges in diagnosing bovine tuberculosis through surveillance and characterization of Mycobacterium species in slaughtered cattle in Kolkata.
hsp65
Abattoir
Duplex PCR
Nontuberculous Mycobacteria
Zoonotic bovine tuberculosis
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
18 Oct 2024
18 Oct 2024
Historique:
received:
28
05
2024
accepted:
09
09
2024
medline:
19
10
2024
pubmed:
19
10
2024
entrez:
18
10
2024
Statut:
epublish
Résumé
Tuberculosis in cattle is caused by Mycobacterium tuberculosis complex (MTBC) species. Apart from MTBC, different Nontuberculous Mycobacteria (NTM) species have also been isolated from cattle. The presence of NTM infection in bovines makes the diagnosis of bovine tuberculosis (bTB) a cumbersome task. Therefore, a cross sectional study was conducted to isolate and characterize different Mycobacterium spp. from a slaughterhouse situated in Kolkata, a city in the eastern part of India. Out of 258 morbid samples, 98 isolates were found to be positive for bacterial growth, and 35% (n = 34) were positive for Mycobacterium. 94% of Mycobacterial cultural isolates were NTM (n = 32), and the rest (n = 2) were found to be MTBC. Species-level identification of the isolates by hsp65 sequencing revealed that out of 32 isolates, 24 were M. fortuitum, three were M. abscessus, two each were M. chelonae and M. parascrofulaceum, and one was M. novocastrense. A phylogenetic tree with partial hsp65 gene sequences was also constructed to determine the relatedness of the unknown isolates to the reference strains. Both NTM species and MTBCs were identified from TB-like lesions in cattle that were slaughtered at the Kolkata abattoir. This discovery may indicate that NTM contributes to the development of lesions in cattle. Also, we recommend implication of more specific diagnostic tests for bTB.
Sections du résumé
BACKGROUND
BACKGROUND
Tuberculosis in cattle is caused by Mycobacterium tuberculosis complex (MTBC) species. Apart from MTBC, different Nontuberculous Mycobacteria (NTM) species have also been isolated from cattle. The presence of NTM infection in bovines makes the diagnosis of bovine tuberculosis (bTB) a cumbersome task. Therefore, a cross sectional study was conducted to isolate and characterize different Mycobacterium spp. from a slaughterhouse situated in Kolkata, a city in the eastern part of India.
RESULTS
RESULTS
Out of 258 morbid samples, 98 isolates were found to be positive for bacterial growth, and 35% (n = 34) were positive for Mycobacterium. 94% of Mycobacterial cultural isolates were NTM (n = 32), and the rest (n = 2) were found to be MTBC. Species-level identification of the isolates by hsp65 sequencing revealed that out of 32 isolates, 24 were M. fortuitum, three were M. abscessus, two each were M. chelonae and M. parascrofulaceum, and one was M. novocastrense. A phylogenetic tree with partial hsp65 gene sequences was also constructed to determine the relatedness of the unknown isolates to the reference strains.
CONCLUSION
CONCLUSIONS
Both NTM species and MTBCs were identified from TB-like lesions in cattle that were slaughtered at the Kolkata abattoir. This discovery may indicate that NTM contributes to the development of lesions in cattle. Also, we recommend implication of more specific diagnostic tests for bTB.
Identifiants
pubmed: 39425195
doi: 10.1186/s12917-024-04272-9
pii: 10.1186/s12917-024-04272-9
doi:
Substances chimiques
Chaperonin 60
0
Bacterial Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
478Subventions
Organisme : Department of Biotechnology, Ministry of Science and Technology, Government of India
ID : BT/ADV/Bovine Tuberculosis/2018
Organisme : Department of Biotechnology, Ministry of Science and Technology, Government of India
ID : BT/ADV/Bovine Tuberculosis/2018
Organisme : Department of Biotechnology, Ministry of Science and Technology, Government of India
ID : BT/ADV/Bovine Tuberculosis/2018
Organisme : Department of Biotechnology, Ministry of Science and Technology, Government of India
ID : BT/ADV/Bovine Tuberculosis/2018
Organisme : Department of Biotechnology, Ministry of Science and Technology, Government of India
ID : BT/ADV/Bovine Tuberculosis/2018
Organisme : Department of Biotechnology, Ministry of Science and Technology, Government of India
ID : BT/ADV/Bovine Tuberculosis/2018
Informations de copyright
© 2024. The Author(s).
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