Sex Differences in Pancreatic β-Cell Physiology and Glucose Homeostasis in C57BL/6J Mice.
beta-cell mass
diabetes
insulin secretion
islet
sex differences
sexual dimorphism
Journal
Journal of the Endocrine Society
ISSN: 2472-1972
Titre abrégé: J Endocr Soc
Pays: United States
ID NLM: 101697997
Informations de publication
Date de publication:
02 Aug 2023
02 Aug 2023
Historique:
received:
18
03
2023
medline:
24
10
2023
pubmed:
24
10
2023
entrez:
24
10
2023
Statut:
epublish
Résumé
The importance of sexual dimorphism has been highlighted in recent years since the National Institutes of Health's mandate on considering sex as a biological variable. Although recent studies have taken strides to study both sexes side by side, investigations into the normal physiological differences between males and females are limited. In this study, we aimed to characterized sex-dependent differences in glucose metabolism and pancreatic β-cell physiology in normal conditions using C57BL/6J mice, the most common mouse strain used in metabolic studies. Here, we report that female mice have improved glucose and insulin tolerance associated with lower nonfasted blood glucose and insulin levels compared with male mice at 3 and 6 months of age. Both male and female animals show β-cell mass expansion from embryonic day 17.5 to adulthood, and no sex differences were observed at embryonic day 17.5, newborn, 1 month, or 3 months of age. However, 6-month-old males displayed increased β-cell mass in response to insulin resistance compared with littermate females. Molecularly, we uncovered sexual dimorphic alterations in the protein levels of nutrient sensing proteins O-GlcNAc transferase and mTOR, as well as differences in glucose-stimulus coupling mechanisms that may underlie the differences in sexually dimorphic β-cell physiology observed in C57BL/6J mice.
Identifiants
pubmed: 37873500
doi: 10.1210/jendso/bvad099
pii: bvad099
pmc: PMC10590649
doi:
Banques de données
figshare
['10.6084/m9.figshare.23712954.v2']
Types de publication
Journal Article
Langues
eng
Pagination
bvad099Subventions
Organisme : NIDDK NIH HHS
ID : F31 DK131860
Pays : United States
Organisme : NIDDK NIH HHS
ID : R56 DK131447
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM140936
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL166142
Pays : United States
Informations de copyright
© The Author(s) 2023. Published by Oxford University Press on behalf of the Endocrine Society.
Références
Cell Cycle. 2012 May 15;11(10):1892-902
pubmed: 22544327
Physiol Rep. 2018 Mar;6(5):
pubmed: 29536672
Diabetes. 2018 May;67(5):872-884
pubmed: 29432124
Diabetologia. 2020 Feb;63(2):324-337
pubmed: 31773193
J Endocrinol. 2019 Jan 1;:
pubmed: 30601759
Obesity (Silver Spring). 2012 Oct;20(10):1966-73
pubmed: 22282046
Genomics Proteomics Bioinformatics. 2021 Jun;19(3):408-422
pubmed: 34571259
J Clin Invest. 2014 Oct;124(10):4395-410
pubmed: 25180600
Nat Commun. 2017 Jul 12;8:16014
pubmed: 28699639
J Biol Chem. 2018 Mar 30;293(13):4735-4751
pubmed: 29378845
Endocrinology. 2016 Feb;157(2):560-9
pubmed: 26653569
Genome Biol. 2014 Dec 03;15(12):522
pubmed: 25517766
J Clin Invest. 2001 Dec;108(11):1631-8
pubmed: 11733558
PLoS One. 2017 Jul 28;12(7):e0182256
pubmed: 28753672
J Diabetes Res. 2020 Dec 16;2020:8872639
pubmed: 33457426
Am J Physiol Endocrinol Metab. 2015 Apr 1;308(7):E573-82
pubmed: 25628421
Endocrinology. 2022 Oct 11;163(11):
pubmed: 36130190
Mol Metab. 2017 Jul 08;6(9):943-957
pubmed: 28951820
Endocr Res. 2016 Aug;41(3):167-79
pubmed: 26865180
Sci Rep. 2017 Aug 10;7(1):7744
pubmed: 28798351
J Womens Health (Larchmt). 2020 Jun;29(6):858-864
pubmed: 31971851
Front Endocrinol (Lausanne). 2022 Jan 17;12:780300
pubmed: 35111136
Children (Basel). 2021 Oct 26;8(11):
pubmed: 34828683
Diabetes. 2016 Sep;65(9):2700-10
pubmed: 27284112
Am J Physiol Endocrinol Metab. 2018 Mar 1;314(3):E274-E286
pubmed: 29138225
Diabetes. 2021 Nov;70(11):2608-2625
pubmed: 34462257
Mol Metab. 2018 Sep;15:82-91
pubmed: 29891438
Diabet Med. 2021 Dec;38(12):e14711
pubmed: 34614258
Am J Physiol Endocrinol Metab. 2002 Aug;283(2):E241-50
pubmed: 12110528
Expert Rev Proteomics. 2013 Aug;10(4):365-80
pubmed: 23992419
Nat Rev Endocrinol. 2012 Feb 14;8(6):342-51
pubmed: 22330739
Cell Rep. 2015 Dec 22;13(11):2527-2538
pubmed: 26673325
Obesity (Silver Spring). 2014 Oct;22(10):2147-55
pubmed: 24942674
Mol Metab. 2021 Nov;53:101260
pubmed: 34023484
Diabetologia. 2010 Nov;53(11):2369-79
pubmed: 20628728
Mol Aspects Med. 2015 Apr;42:19-41
pubmed: 25542976
Biol Sex Differ. 2015 Sep 03;6:14
pubmed: 26339468
J Clin Invest. 2015 Oct 1;125(10):3831-46
pubmed: 26389675
Mol Metab. 2023 Mar;69:101678
pubmed: 36690328
Diabetologia. 2019 Aug;62(8):1445-1452
pubmed: 31177313
J Neurosci Methods. 2022 Jan 1;365:109384
pubmed: 34634282
Diabetes. 2017 Aug;66(8):2150-2162
pubmed: 28546423
J Biol Chem. 2019 Aug 30;294(35):13040-13050
pubmed: 31300553
JCI Insight. 2022 Nov 8;7(21):
pubmed: 36107617
Front Endocrinol (Lausanne). 2015 Jan 19;5:241
pubmed: 25646091
Diabetes Care. 2015 Nov;38(11):e176-7
pubmed: 26384388
JCI Insight. 2021 Jul 8;6(13):
pubmed: 34032632
Am J Physiol Regul Integr Comp Physiol. 2018 Dec 1;315(6):R1220-R1231
pubmed: 30303709
J Diabetes Investig. 2018 Nov;9(6):1283-1287
pubmed: 29489067
Nat Commun. 2022 Feb 8;13(1):735
pubmed: 35136059
Adv Exp Med Biol. 2015;803:371-85
pubmed: 25833510
Diabetologia. 2013 May;56(5):1129-39
pubmed: 23423668
Dialogues Clin Neurosci. 2016 Dec;18(4):459-464
pubmed: 28179817
Cell Rep. 2020 May 5;31(5):107609
pubmed: 32375037
Diabetologia. 2020 Mar;63(3):453-461
pubmed: 31754750