Characterization of thraustochytrid-specific sterol
DGAT
diacylglycerol
sterol ester
sterol o-acyltransferase
sterols
thraustochytrid
Journal
Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801
Informations de publication
Date de publication:
29 Nov 2023
29 Nov 2023
Historique:
medline:
30
11
2023
pubmed:
24
10
2023
entrez:
24
10
2023
Statut:
ppublish
Résumé
Since the global market for sterols and vitamin D are grown with a high compound annual growth rate, a sustainable source of these compounds is required to keep up with the increasing demand. Thraustochytrid is a marine oleaginous microorganism that can synthesize several sterols, which are stored as SE in lipid droplets. DGAT2C is an unconventional SE synthase specific to thraustochytrids. Although the primary structure of DGAT2C shows high similarities with that of DGAT, DGAT2C utilizes sterol as an acceptor substrate instead of diacylglycerol. In this study, we examined more detailed enzymatic properties, intracellular localization, and structure-activity relationship of DGAT2C. Furthermore, we successfully developed a method to increase sterol and provitamin D3 productivity of thraustochytrid by more than threefold in the process of elucidating the function of the DGAT2C-specific N-terminal region. Our findings could lead to sustainable sterol and vitamin D production using thraustochytrid.
Identifiants
pubmed: 37874286
doi: 10.1128/aem.01001-23
pmc: PMC10686087
doi:
Substances chimiques
Sterols
0
Sterol O-Acyltransferase
EC 2.3.1.26
Vitamin D
1406-16-2
Diacylglycerol O-Acyltransferase
EC 2.3.1.20
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0100123Subventions
Organisme : MEXT | Japan Society for the Promotion of Science (JSPS)
ID : JP15K18740, 21K06034
Organisme : MEXT | Japan Science and Technology Agency (JST)
ID : JST Global Science Campus
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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