PAPγ associates with PAXT nuclear exosome to control the abundance of PROMPT ncRNAs.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
24 10 2023
24 10 2023
Historique:
received:
06
05
2022
accepted:
17
10
2023
medline:
27
10
2023
pubmed:
25
10
2023
entrez:
24
10
2023
Statut:
epublish
Résumé
Pervasive transcription of the human genome generates an abundance of RNAs that must be processed and degraded. The nuclear RNA exosome is the main RNA degradation machinery in the nucleus. However, nuclear exosome must be recruited to its substrates by targeting complexes, such as NEXT or PAXT. By proteomic analysis, we identify additional subunits of PAXT, including many orthologs of MTREC found in S. pombe. In particular, we show that polyA polymerase gamma (PAPγ) associates with PAXT. Genome-wide mapping of the binding sites of ZFC3H1, RBM27 and PAPγ shows that PAXT is recruited to the TSS of hundreds of genes. Loss of ZFC3H1 abolishes recruitment of PAXT subunits including PAPγ to TSSs and concomitantly increases the abundance of PROMPTs at the same sites. Moreover, PAPγ, as well as MTR4 and ZFC3H1, is implicated in the polyadenylation of PROMPTs. Our results thus provide key insights into the direct targeting of PROMPT ncRNAs by PAXT at their genomic sites.
Identifiants
pubmed: 37875486
doi: 10.1038/s41467-023-42620-9
pii: 10.1038/s41467-023-42620-9
pmc: PMC10598014
doi:
Substances chimiques
Exosome Multienzyme Ribonuclease Complex
EC 3.1.-
RNA
63231-63-0
RNA, Untranslated
0
Polynucleotide Adenylyltransferase
EC 2.7.7.19
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6745Informations de copyright
© 2023. Springer Nature Limited.
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