MALDI Mass Spectrometry Imaging and Semi-Quantification of Topically Delivered Lactic Acid.
Franz cell
cationic xylene
chemical peels
derivatization
isotope labeling
stratum corneum
trichloroacetic acid
Journal
Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
ISSN: 1600-0846
Titre abrégé: Skin Res Technol
Pays: England
ID NLM: 9504453
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
23
06
2023
accepted:
19
09
2023
medline:
27
10
2023
pubmed:
26
10
2023
entrez:
26
10
2023
Statut:
ppublish
Résumé
Lactic acid is a common active ingredient in many topical skincare products; however, measuring its delivery into the skin is challenging due to the presence of a large level of endogenous lactic acid. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was used to quantitatively and qualitatively measure the delivery of lactic acid into the skin from a range of topical skincare products. Porcine skin samples were treated with various skincare products containing lactic acid. After 24 h, skin samples were sectioned and treated via H&E staining or prepared for MALDI-MSI using chemical derivatization. Samples were then analyzed by MALDI-MSI imaging to obtain lactic acid distribution in the entire skin section. Due to the high level of endogenous lactic acid in the skin, a "triple isotope" of lactic acid (L-Lactic acid- The combination of L-Lactic acid-
Sections du résumé
BACKGROUND
BACKGROUND
Lactic acid is a common active ingredient in many topical skincare products; however, measuring its delivery into the skin is challenging due to the presence of a large level of endogenous lactic acid. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) was used to quantitatively and qualitatively measure the delivery of lactic acid into the skin from a range of topical skincare products.
MATERIALS AND METHODS
METHODS
Porcine skin samples were treated with various skincare products containing lactic acid. After 24 h, skin samples were sectioned and treated via H&E staining or prepared for MALDI-MSI using chemical derivatization. Samples were then analyzed by MALDI-MSI imaging to obtain lactic acid distribution in the entire skin section.
RESULTS
RESULTS
Due to the high level of endogenous lactic acid in the skin, a "triple isotope" of lactic acid (L-Lactic acid-
CONCLUSIONS
CONCLUSIONS
The combination of L-Lactic acid-
Identifiants
pubmed: 37881041
doi: 10.1111/srt.13485
pmc: PMC10579629
doi:
Substances chimiques
Lactic Acid
33X04XA5AT
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13485Informations de copyright
© 2023 Colgate Palmolive and The Authors. Skin Research and Technology published by John Wiley & Sons Ltd.
Références
Int J Dermatol. 2019 Mar;58(3):374-379
pubmed: 30270529
Front Med (Lausanne). 2021 Feb 12;8:617068
pubmed: 33681250
Arch Klin Exp Dermatol. 1957;204(2):140-50
pubmed: 13425566
Australas J Dermatol. 2018 Aug;59(3):171-181
pubmed: 29064096
Dermatol Ther. 2011 Sep-Oct;24(5):471-6
pubmed: 22353153
Anal Bioanal Chem. 2018 Jul;410(17):4015-4038
pubmed: 29682685
Bioanalysis. 2012 Sep;4(17):2095-8
pubmed: 23013389
Dermatol Res Pract. 2021 Aug 30;2021:3085670
pubmed: 34504524
Anal Chem. 1997 Dec 1;69(23):4751-60
pubmed: 9406525
Dermatol Surg. 2005 Feb;31(2):149-54; discussion 154
pubmed: 15762205
Anal Bioanal Chem. 2022 Aug;414(19):5781-5791
pubmed: 35650447
J Proteomics. 2012 Aug 30;75(16):4952-4961
pubmed: 22842155
J Am Soc Mass Spectrom. 2015 Oct;26(10):1713-21
pubmed: 26115969
J Dermatol. 2018 Sep;45(9):1044-1052
pubmed: 29863755
Anal Bioanal Chem. 2018 Apr;410(11):2815-2828
pubmed: 29546543
J Labelled Comp Radiopharm. 2013 Jul-Aug;56(9-10):441-6
pubmed: 24285517
J Pharm Biomed Anal. 2013 Jun;80:107-15
pubmed: 23542733
Clin Dermatol. 2008 Mar-Apr;26(2):200-8
pubmed: 18472061
Curr Pharm Biotechnol. 2020;21(7):566-577
pubmed: 31916515
Biomed Mass Spectrom. 1983 Aug;10(8):458-62
pubmed: 6616022
Chem Commun (Camb). 2016 Aug 14;52(63):9801-4
pubmed: 27419250
Anal Chem. 2018 May 15;90(10):6051-6058
pubmed: 29668267
Cell Tissue Res. 2015 Apr;360(1):87-107
pubmed: 25604842