Neuronal types in the mouse amygdala and their transcriptional response to fear conditioning.
Journal
Nature neuroscience
ISSN: 1546-1726
Titre abrégé: Nat Neurosci
Pays: United States
ID NLM: 9809671
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
19
07
2022
accepted:
20
09
2023
medline:
4
12
2023
pubmed:
27
10
2023
entrez:
26
10
2023
Statut:
ppublish
Résumé
The amygdala is a brain region primarily associated with emotional response. The use of genetic markers and single-cell transcriptomics can provide insights into behavior-associated cell state changes. Here we present a detailed cell-type taxonomy of the adult mouse amygdala during fear learning and memory consolidation. We perform single-cell RNA sequencing on naïve and fear-conditioned mice, identify 130 neuronal cell types and validate their spatial distributions. A subset of all neuronal types is transcriptionally responsive to fear learning and memory retrieval. The activated engram cells upregulate activity-response genes and coordinate the expression of genes associated with neurite outgrowth, synaptic signaling, plasticity and development. We identify known and previously undescribed candidate genes responsive to fear learning. Our molecular atlas may be used to generate hypotheses to unveil the neuron types and neural circuits regulating the emotional component of learning and memory.
Identifiants
pubmed: 37884748
doi: 10.1038/s41593-023-01469-3
pii: 10.1038/s41593-023-01469-3
pmc: PMC10689239
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2237-2249Subventions
Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
ID : 852786
Organisme : Human Frontier Science Program (HFSP)
ID : CDA-0039/2019-C
Organisme : Israel Science Foundation (ISF)
ID : 2028912
Informations de copyright
© 2023. The Author(s).
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