Biodegradation of PET by the membrane-anchored PET esterase from the marine bacterium Rhodococcus pyridinivorans P23.
Journal
Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179
Informations de publication
Date de publication:
27 10 2023
27 10 2023
Historique:
received:
14
04
2023
accepted:
16
10
2023
medline:
30
10
2023
pubmed:
28
10
2023
entrez:
27
10
2023
Statut:
epublish
Résumé
Evidence for microbial biodegradation of polyethylene terephthalate (PET) has been reported, but little is known about the PET biodegradation process and molecular mechanism by marine microorganisms. Here, we show the biodegradation of PET by the membrane-anchored PET esterase from the marine bacterium Rhodococcus pyridinivorans P23, elucidate the properties of this enzyme, and propose the PET biodegradation by this strain in biofilm. We identify the PET-degrading enzyme dubbed PET esterase through activity tracking. In addition to depolymerizing PET, it hydrolyzes MHET into TPA under acid conditions. We prove that it is a low and constitutively transcribed, membrane-anchored protein displayed on the cell surface. Furthermore, we also investigate the microbial groups possessing PET esterase coupled with the TPA degradation pathway, mainly in the phyla Proteobacteria and Actinobacteriota. Clarification of the microbial PET biodegradation in the marine environment will contribute to the understanding of bioremediation of marine PET pollution.
Identifiants
pubmed: 37891241
doi: 10.1038/s42003-023-05470-1
pii: 10.1038/s42003-023-05470-1
pmc: PMC10611731
doi:
Substances chimiques
Polyethylene Terephthalates
0
Esterases
EC 3.1.-
Pyridinolcarbamate
81R511UV73
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1090Informations de copyright
© 2023. The Author(s).
Références
Front Bioeng Biotechnol. 2022 Jul 21;10:930140
pubmed: 35935485
Nat Biotechnol. 2015 Jun;33(6):623-30
pubmed: 26006009
Science. 2018 Apr 6;360(6384):28-29
pubmed: 29622640
Sci Total Environ. 2018 Sep 15;636:20-29
pubmed: 29702399
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D115-9
pubmed: 14681372
Nat Rev Microbiol. 2016 Aug 11;14(9):563-75
pubmed: 27510863
Sci Total Environ. 2018 Sep 1;634:811-820
pubmed: 29653425
Nat Methods. 2013 Jun;10(6):563-9
pubmed: 23644548
Biodegradation. 2008 Nov;19(6):851-8
pubmed: 18401686
Science. 2009 Jan 2;323(5910):133-8
pubmed: 19023044
Biophys J. 2018 Mar 27;114(6):1302-1312
pubmed: 29590588
Chem Rev. 2023 May 10;123(9):5612-5701
pubmed: 36916764
Chembiochem. 2018 Jul 16;19(14):1471-1475
pubmed: 29603535
Microbes Environ. 2009;24(1):39-42
pubmed: 21566352
Mar Pollut Bull. 2019 Jan;138:549-560
pubmed: 30660306
PLoS One. 2016 Aug 03;11(8):e0159289
pubmed: 27487037
Environ Microbiol. 2023 Jul 8;:
pubmed: 37421171
Biotechnol Rep (Amst). 2021 Nov 18;32:e00689
pubmed: 34987981
Nat Commun. 2023 Jul 28;14(1):4556
pubmed: 37507390
Nat Genet. 2000 May;25(1):25-9
pubmed: 10802651
Sci Prog. 2018 Sep 1;101(3):207-260
pubmed: 30025551
J Biol Chem. 2004 Feb 20;279(8):6815-23
pubmed: 14617621
Nat Commun. 2018 Jan 26;9(1):382
pubmed: 29374183
Front Microbiol. 2019 Oct 01;10:2187
pubmed: 31632361
Nat Commun. 2020 May 12;11(1):2372
pubmed: 32398678
Nat Commun. 2020 Nov 13;11(1):5790
pubmed: 33188179
Environ Sci Technol. 2016 Mar 15;50(6):3239-46
pubmed: 26905979
Sci Total Environ. 2018 Nov 1;640-641:637-645
pubmed: 29870939
Sci Total Environ. 2022 Feb 20;808:152107
pubmed: 34864034
Microbiol Spectr. 2021 Dec 22;9(3):e0097621
pubmed: 34730414
FEMS Microbiol Ecol. 2020 Nov 25;96(12):
pubmed: 33181829
Nat Methods. 2008 Jul;5(7):621-8
pubmed: 18516045
Nat Commun. 2022 Jul 6;13(1):3884
pubmed: 35794126
Environ Res. 2022 Apr 15;206:112542
pubmed: 34929185
PLoS One. 2023 Mar 8;18(3):e0281596
pubmed: 36888681
Appl Microbiol Biotechnol. 2018 Sep;102(18):7669-7678
pubmed: 29992436
Nature. 2020 Apr;580(7802):216-219
pubmed: 32269349
Nucleic Acids Res. 2000 Jan 1;28(1):33-6
pubmed: 10592175
J Hazard Mater. 2021 Aug 15;416:125928
pubmed: 34489083
Nucleic Acids Res. 2019 May 21;47(9):4442-4448
pubmed: 31081040
Appl Environ Microbiol. 2018 Apr 2;84(8):
pubmed: 29427431
Front Microbiol. 2020 Feb 13;11:114
pubmed: 32117139
J Hazard Mater. 2019 Dec 15;380:120899
pubmed: 31326835
Science. 2016 Mar 11;351(6278):1196-9
pubmed: 26965627
BMC Bioinformatics. 2010 Mar 08;11:119
pubmed: 20211023
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D277-80
pubmed: 14681412
Sci Total Environ. 2019 Feb 10;650(Pt 2):1913-1922
pubmed: 30286357
Appl Microbiol Biotechnol. 2015 Nov;99(21):8857-74
pubmed: 26318446
Front Microbiol. 2019 Apr 25;10:865
pubmed: 31073297
Mar Pollut Bull. 2011 Jan;62(1):197-200
pubmed: 21093883
Environ Sci Technol. 2013 Jul 2;47(13):7137-46
pubmed: 23745679