T Cell Antigen Recognition and Discrimination by Electrochemiluminescence Imaging.

Antigen Recognition and Discrimination Electrochemiluminescence Immune Synapse Label-Free Imaging T Cell Receptor

Journal

Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543

Informations de publication

Date de publication:
11 Dec 2023
Historique:
received: 11 10 2023
medline: 7 12 2023
pubmed: 31 10 2023
entrez: 30 10 2023
Statut: ppublish

Résumé

Adoptive T lymphocyte (T cell) transfer and tumour-specific peptide vaccines are innovative cancer therapies. An accurate assessment of the specific reactivity of T cell receptors (TCRs) to tumour antigens is required because of the high heterogeneity of tumour cells and the immunosuppressive tumour microenvironment. In this study, we report a label-free electrochemiluminescence (ECL) imaging approach for recognising and discriminating between TCRs and tumour-specific antigens by imaging the immune synapses of T cells. Various T cell stimuli, including agonistic antibodies, auxiliary molecules, and tumour-specific antigens, were modified on the electrode's surface to allow for their interaction with T cells bearing different TCRs. The formation of immune synapses activated by specific stimuli produced a negative (shadow) ECL image, from which T cell antigen recognition and discrimination were evaluated by analysing the spreading area and the recognition intensity of T cells. This approach provides an easy way to assess TCR-antigen specificity and screen both of them for immunotherapies.

Identifiants

pubmed: 37903724
doi: 10.1002/anie.202314588
doi:

Substances chimiques

Receptors, Antigen, T-Cell 0
Antigens, Neoplasm 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202314588

Subventions

Organisme : National Natural Science Foundation of China
ID : 22125405
Organisme : National Natural Science Foundation of China
ID : 22074131

Informations de copyright

© 2023 Wiley-VCH GmbH.

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Auteurs

Yajuan Yan (Y)

Key Laboratory of Excited-State Materials of Zhejiang Province, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.

Ping Zhou (P)

Key Laboratory of Excited-State Materials of Zhejiang Province, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.

Lurong Ding (L)

Key Laboratory of Excited-State Materials of Zhejiang Province, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.

Wei Hu (W)

Kidney Disease Center, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310058, China.

Wei Chen (W)

Department of Cardiology of the Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou, 310058, China.
Liangzhu Laboratory, Zhejiang University, Hangzhou, 311121, China.
Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Ministry of Education Frontier Science Center for Brain Science & Brain-machine Integration, State Key Laboratory for Modern Optical Instrumentation, Key Laboratory for Biomedical Engineering of the Ministry of Education, College of Biomedical Engineering and Instrument Science, Zhejiang University, Hangzhou, Zhejiang 310012, China.

Bin Su (B)

Key Laboratory of Excited-State Materials of Zhejiang Province, Department of Chemistry, Zhejiang University, Hangzhou, 310058, China.

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