HIV-1 diverts cortical actin for particle assembly and release.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
31 10 2023
31 10 2023
Historique:
received:
06
12
2022
accepted:
25
09
2023
medline:
2
11
2023
pubmed:
1
11
2023
entrez:
1
11
2023
Statut:
epublish
Résumé
Enveloped viruses assemble and bud from the host cell membranes. Any role of cortical actin in these processes have often been a source of debate. Here, we assessed if cortical actin was involved in HIV-1 assembly in infected CD4 T lymphocytes. Our results show that preventing actin branching not only increases HIV-1 particle release but also the number of individual HIV-1 Gag assembly clusters at the T cell plasma membrane. Indeed, in infected T lymphocytes and in in vitro quantitative model systems, we show that HIV-1 Gag protein prefers areas deficient in F-actin for assembling. Finally, we found that the host factor Arpin, an inhibitor of Arp2/3 branched actin, is recruited at the membrane of infected T cells and it can associate with the viral Gag protein. Altogether, our data show that, for virus assembly and particle release, HIV-1 prefers low density of cortical actin and may favor local actin debranching by subverting Arpin.
Identifiants
pubmed: 37907528
doi: 10.1038/s41467-023-41940-0
pii: 10.1038/s41467-023-41940-0
pmc: PMC10618566
doi:
Substances chimiques
Actins
0
Gene Products, gag
0
Viral Proteins
0
gag Gene Products, Human Immunodeficiency Virus
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6945Informations de copyright
© 2023. The Author(s).
Références
J Virol. 2007 Nov;81(22):12596-607
pubmed: 17728233
Cell Res. 2019 Jun;29(6):432-445
pubmed: 30971746
PLoS One. 2013;8(1):e53138
pubmed: 23308151
Curr Biol. 2001 Oct 30;11(21):1645-55
pubmed: 11696321
J Biol Chem. 1994 May 27;269(21):14869-71
pubmed: 8195116
Mol Biol Cell. 2015 Mar 1;26(5):887-900
pubmed: 25568343
Cancer Res. 2020 Jul 1;80(13):2804-2817
pubmed: 32376602
Bioinformatics. 2014 Sep 1;30(17):2532-3
pubmed: 24825612
J Virol. 2014 Jul;88(14):7904-14
pubmed: 24789789
Eur Phys J E Soft Matter. 2014 May;37(5):42
pubmed: 24859281
Retrovirology. 2005 Aug 04;2:48
pubmed: 16080789
J Virol. 2014 Jul;88(14):7893-903
pubmed: 24789788
J Cell Biol. 2018 Aug 6;217(8):2911-2929
pubmed: 29921601
Front Cell Dev Biol. 2021 May 03;9:625719
pubmed: 34012961
J Cell Sci. 2002 Jan 15;115(Pt 2):433-43
pubmed: 11839793
J Virol. 2014 Jun;88(12):6906-21
pubmed: 24696479
J Vis Exp. 2015 Dec 17;(106):e53749
pubmed: 26709554
Nat Methods. 2015 Nov;12(11):1065-71
pubmed: 26344046
J Biol Chem. 2011 Aug 12;286(32):28498-510
pubmed: 21705339
BMC Biol. 2013 Aug 02;11:89
pubmed: 23915020
Curr Biol. 2014 Jan 6;24(1):R31-R33
pubmed: 24405676
Cell. 2001 Oct 5;107(1):55-65
pubmed: 11595185
J Cell Biol. 2002 Dec 23;159(6):993-1004
pubmed: 12499356
Cell Host Microbe. 2007 May 17;1(3):227-40
pubmed: 18005701
Pathogens. 2022 Jan 03;11(1):
pubmed: 35056004
Elife. 2021 Jun 11;10:
pubmed: 34114563
Viruses. 2019 Jan 16;11(1):
pubmed: 30654596
Biophys J. 2005 Dec;89(6):4029-42
pubmed: 16199500
Viruses. 2021 Feb 01;13(2):
pubmed: 33535486
J Mol Biol. 2017 Jan 20;429(2):237-248
pubmed: 27939292
J Cell Sci. 2013 Mar 1;126(Pt 5):1049-58
pubmed: 23620508
PLoS Pathog. 2010 Nov 24;6(11):e1001173
pubmed: 21124872
Nat Commun. 2020 Apr 3;11(1):1649
pubmed: 32245998
Commun Biol. 2021 Jun 25;4(1):799
pubmed: 34172833
Sci Rep. 2015 Jun 29;5:11454
pubmed: 26118385
Life Sci. 2020 Nov 15;261:118468
pubmed: 32961232
Virus Res. 2009 Aug;143(2):162-76
pubmed: 19383519
J Cell Sci. 2022 Oct 1;135(19):
pubmed: 36074045
J Virol. 2015 Aug;89(16):8162-81
pubmed: 26018170
PLoS One. 2014 Jan 31;9(1):e87700
pubmed: 24498171
Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2138-2143
pubmed: 29439200
Nat Commun. 2015 Jan 19;6:6027
pubmed: 25597399
Methods Mol Biol. 2009;586:71-87
pubmed: 19768425
Nat Cell Biol. 2022 Jul;24(7):1077-1087
pubmed: 35739319
Sci Rep. 2016 Dec 23;6:39332
pubmed: 28008947
Sci Adv. 2019 Oct 02;5(10):eaaw8651
pubmed: 31616784
Biophys J. 2023 Jun 6;122(11):2216-2229
pubmed: 36632034
J Cell Sci. 2003 Jun 15;116(Pt 12):2577-83
pubmed: 12734400
J Cell Sci. 2011 Mar 1;124(Pt 5):679-83
pubmed: 21321325
Proc Natl Acad Sci U S A. 1995 Mar 14;92(6):2026-30
pubmed: 7892219
Cell. 2016 Feb 11;164(4):695-709
pubmed: 26830877
Nat Commun. 2022 Feb 2;13(1):628
pubmed: 35110533
Science. 2009 Nov 27;326(5957):1208-12
pubmed: 19965462
Nature. 2008 Jul 10;454(7201):236-40
pubmed: 18500329
Cell. 2019 May 2;177(4):806-819
pubmed: 31051105
Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14889-94
pubmed: 15465916
Viruses. 2018 Feb 05;10(2):
pubmed: 29401736
Phys Rev E Stat Nonlin Soft Matter Phys. 2008 Nov;78(5 Pt 1):051903
pubmed: 19113151
J Virol. 2007 Jun;81(11):5547-60
pubmed: 17360745
Curr Biol. 2010 May 11;20(9):861-7
pubmed: 20362448
Elife. 2014 Mar 18;3:e01671
pubmed: 24642407
PLoS Pathog. 2022 Jan 24;18(1):e1010264
pubmed: 35073379
Curr Biol. 2013 Jun 17;23(12):1037-45
pubmed: 23727094
J Phys Condens Matter. 2015 Jul 15;27(27):273103
pubmed: 26061624
Trends Cell Biol. 2006 Oct;16(10):522-9
pubmed: 16949823
Chem Biol. 2013 May 23;20(5):701-12
pubmed: 23623350
Methods. 2018 May 1;140-141:112-118
pubmed: 29410223
Nature. 2013 Nov 14;503(7475):281-4
pubmed: 24132237
Trends Cell Biol. 2020 Jul;30(7):556-565
pubmed: 32278656
Sci Rep. 2018 Nov 2;8(1):16283
pubmed: 30389967