A dual-mode strategy based on β-galactosidase and target-induced DNA polymerase protection for transcription factor detection using colorimetry and a glucose meter.

Transcription Factors / genetics Colorimetry DNA-Directed DNA Polymerase beta-Galactosidase Glucose

Journal

The Analyst
ISSN: 1364-5528
Titre abrégé: Analyst
Pays: England
ID NLM: 0372652

Informations de publication

Date de publication:
20 Nov 2023
Historique:
medline: 21 11 2023
pubmed: 1 11 2023
entrez: 1 11 2023
Statut: epublish

Résumé

In this work, we report a novel dual-mode method for the highly specific and sensitive detection of transcription factors (TFs)

Identifiants

DOI: 10.1039/d3an01414b PMID: 37909394
pubmed: 37909394
doi: 10.1039/d3an01414b
doi:

Substances chimiques

Transcription Factors 0
DNA-Directed DNA Polymerase EC 2.7.7.7
beta-Galactosidase EC 3.2.1.23
Glucose IY9XDZ35W2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

6078-6086

Auteurs

Xinmei Qian (X)

Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education, Hunan University of Science and Technology, Xiangtan 411201, China. keqindeng@hnust.edu.cn.
Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Provincial Key Laboratory of Advanced Materials for New Energy Storage and Conversion, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.

Heng Zhang (H)

Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Provincial Key Laboratory of Advanced Materials for New Energy Storage and Conversion, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.

Mingyu Zheng (M)

Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education, Hunan University of Science and Technology, Xiangtan 411201, China. keqindeng@hnust.edu.cn.

Chunxiang Li (C)

Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Provincial Key Laboratory of Advanced Materials for New Energy Storage and Conversion, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.

Jinglun Wang (J)

Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, Hunan Provincial Key Laboratory of Advanced Materials for New Energy Storage and Conversion, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, China.

Haowen Huang (H)

Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education, Hunan University of Science and Technology, Xiangtan 411201, China. keqindeng@hnust.edu.cn.

Keqin Deng (K)

Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education, Hunan University of Science and Technology, Xiangtan 411201, China. keqindeng@hnust.edu.cn.
ORCID: 0000-0003-3420-6536

Articles similaires

Expression interplay of genes coding for calcium-binding proteins and transcription factors during the osmotic phase provides insights on salt stress response mechanisms in bread wheat.

Diana Duarte-Delgado, Inci Vogt, Said Dadshani et al.
1.00
Triticum Transcription Factors Gene Expression Regulation, Plant Plant Proteins Salt Stress

Suppression of non-muscle myosin II boosts T cell cytotoxicity against tumors.

Yingyun Yang, Dahan Wen, Feng Lin et al.
1.00
Animals Humans Mice Neoplasms Tumor Microenvironment

Benchmarking and building DNA binding affinity models using allele-specific and allele-agnostic transcription factor binding data.

Xiaoting Li, Lucas A N Melo, Harmen J Bussemaker
1.00
Alleles Benchmarking Transcription Factors Humans Chromatin Immunoprecipitation Sequencing

Genome-wide profiling of soybean WRINKLED1 transcription factor binding sites provides insight into seed storage lipid biosynthesis.

Leonardo Jo, Julie M Pelletier, Robert B Goldberg et al.
1.00
Glycine max Seeds Transcription Factors Gene Expression Regulation, Plant Plant Proteins

Classifications MeSH

questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › A dual-mode strategy based on β-galactosidase...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Titrimétrie › Colorimétrie › A dual-mode strategy based on β-galactosidase...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › DNA nucleotidyltransferases › DNA-directed DNA polymerase › A dual-mode strategy based on β-galactosidase...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Galactosidases › beta-Galactosidase › A dual-mode strategy based on β-galactosidase...
questionsmedicales.fr › Glucides › Sucres › Oses › Hexose › Glucose › A dual-mode strategy based on β-galactosidase...