Prevalence and distribution of subtypes of Blastocystis in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) farmed in Hainan, China.

Prévalence et répartition des sous-types de Blastocystis chez les athérures à longue queue (Atherurus macrourus), les rats des bambous (Rhizomys pruinosus) et les civettes masquées (Paguma larvata) élevés en Chine dans le Hainan.

Journal

Parasite (Paris, France)
ISSN: 1776-1042
Titre abrégé: Parasite
Pays: France
ID NLM: 9437094

Informations de publication

Date de publication:
2023
Historique:
received: 07 09 2023
accepted: 19 10 2023
medline: 6 11 2023
pubmed: 3 11 2023
entrez: 3 11 2023
Statut: ppublish

Résumé

Blastocystis sp. is an important gastrointestinal parasite with global distribution, prevalent in humans, farmed animals, and wildlife. Therefore, this study aimed to investigate the prevalence and genetic diversity of Blastocystis sp. in Asiatic brush-tailed porcupines (Atherurus macrourus), bamboo rats (Rhizomys pruinosus), and masked palm civets (Paguma larvata) in Hainan Province, China. A total of 900 fecal samples were collected from three farmed animal species including 257 porcupines, 360 rats, and 283 civets. Genomic DNA was extracted from each fecal sample and Blastocystis sp. was detected by PCR at the small subunit ribosomal RNA (SSU rRNA) gene. A phylogenetic tree was constructed using the maximum likelihood method. Blastocystis sp. was detected in 47 (5.2%) fecal samples: 12 (4.7%) Asiatic brush-tailed porcupines, 8 (2.2%) bamboo rats, and 27 (9.5%) masked palm civets. Three known Blastocystis sp. subtypes, including ST1, ST4, ST5, and one unnamed subtype (unST), were found in one, 19, 26, and one animal, respectively. Subtypes ST4 and unST were detected in porcupines, ST4 in rats, and ST1 and ST5 in civets. Our results suggest that the three farmed animal species reported in this study could serve as reservoirs for potentially zoonotic Blastocystis sp. subtypes and transmit this parasite to humans, other farmed animals, and wildlife. Prévalence et répartition des sous-types de Blastocystis chez les athérures à longue queue (Atherurus macrourus), les rats des bambous (Rhizomys pruinosus) et les civettes masquées (Paguma larvata) élevés en Chine dans le Hainan. Blastocystis sp. est un parasite gastro-intestinal important avec une distribution mondiale, répandu chez les humains, les animaux d’élevage et la faune. Par conséquent, cette étude visait à étudier la prévalence et la diversité génétique de Blastocystis sp. chez les athérures à longue queue (Atherurus macrourus), les rats des bambous (Rhizomys pruinosus) et les civettes masquées (Paguma larvata) dans la province de Hainan, en Chine. Au total, 900 échantillons fécaux ont été collectés sur ces trois espèces animales d’élevage dont 257 athérures, 360 rats et 283 civettes. L’ADN génomique a été extrait de chaque échantillon fécal et Blastocystis sp. a été détecté par PCR au niveau du gène de la petite sous-unité de l’ARN ribosomal. Un arbre phylogénétique a été construit en utilisant la méthode du maximum de vraisemblance. Blastocystis sp. a été détecté dans 47 (5,2 %) échantillons fécaux : 12 (4,7 %) athérures, 8 (2,2 %) rats et 27 (9,5 %) civettes. Trois sous-types de Blastocystis sp., dont ST1, ST4, ST5 et un sous-type sans nom (unST), ont été trouvés respectivement chez 1, 19, 26 et 1 animal. Les sous-types ST4 et unST ont été détectés chez les athérures, ST4 chez les rats et ST1 et ST5 chez les civettes. Nos résultats suggèrent que les trois espèces animales d’élevage concernées par cette étude pourraient servir de réservoirs à des sous-types potentiellement zoonotiques de Blastocystis sp. et transmettre ce parasite aux humains, à d’autres animaux d’élevage et à la faune.

Autres résumés

Type: Publisher (fre)
Prévalence et répartition des sous-types de Blastocystis chez les athérures à longue queue (Atherurus macrourus), les rats des bambous (Rhizomys pruinosus) et les civettes masquées (Paguma larvata) élevés en Chine dans le Hainan.

Identifiants

pubmed: 37921619
doi: 10.1051/parasite/2023048
pii: parasite230128
pmc: PMC10624160
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

45

Subventions

Organisme : Department of Education Scientific Research Project of Zhejiang (Y202249687)
ID : Y202249687

Informations de copyright

© W. Zhao et al., published by EDP Sciences, 2023.

Références

Parasite. 2021;28:81
pubmed: 34907896
J Eukaryot Microbiol. 2020 Mar;67(2):273-278
pubmed: 31691450
Infect Genet Evol. 2021 Nov;95:105085
pubmed: 34530156
Vet Res Commun. 2021 Dec;45(4):441-445
pubmed: 33987777
PLoS One. 2017 Jan 6;12(1):e0169659
pubmed: 28060901
Res Vet Sci. 2021 Mar;135:260-282
pubmed: 33046256
Int J Parasitol Parasites Wildl. 2020 Jan 30;11:143-148
pubmed: 32071861
Infect Genet Evol. 2014 Mar;22:223-8
pubmed: 23886615
Mol Biol Evol. 2018 Jun 1;35(6):1547-1549
pubmed: 29722887
Acta Trop. 2019 Sep;197:105065
pubmed: 31220434
Trop Biomed. 2018 Jun 1;35(2):586-592
pubmed: 33601834
Vet Parasitol Reg Stud Reports. 2021 Dec;26:100630
pubmed: 34879941
Parasitol Res. 2020 Feb;119(2):465-471
pubmed: 31845021
Parasitol Int. 2016 Dec;65(6 Pt B):780-784
pubmed: 27080248
Environ Sci Pollut Res Int. 2021 Nov;28(43):60308-60328
pubmed: 34528202
Parasit Vectors. 2020 Jul 22;13(1):365
pubmed: 32698882
Microbiol Spectr. 2021 Dec 22;9(3):e0099021
pubmed: 34730381
Parasitol Res. 2020 Jan;119(1):97-104
pubmed: 31735993
Parasite. 2020;27:35
pubmed: 32410727
Infect Genet Evol. 2020 Mar;78:104053
pubmed: 31683006
Animals (Basel). 2021 Mar 05;11(3):
pubmed: 33807707
Parasitology. 2018 Aug;145(9):1228-1234
pubmed: 29338807
Int J Parasitol Parasites Wildl. 2021 Sep 01;16:120-125
pubmed: 34552843
J Eukaryot Microbiol. 2023 Sep 1;:e12998
pubmed: 37658622
Parasit Vectors. 2022 Jan 6;15(1):14
pubmed: 34991712
Parasitol Res. 2011 Jul;109(1):205-12
pubmed: 21210149
Protist. 2019 Nov;170(5):125679
pubmed: 31580985
Front Microbiol. 2020 Feb 25;11:288
pubmed: 32161577
Vet Parasitol. 2013 Sep 1;196(1-2):85-9
pubmed: 23398989
Int J Parasitol Parasites Wildl. 2019 Jun 26;9:298-304
pubmed: 31341768
Parasit Vectors. 2018 Sep 20;11(1):522
pubmed: 30236147
Parasitol Int. 2016 Dec;65(6 Pt B):763-771
pubmed: 27247124
Sci Total Environ. 2022 Feb 1;806(Pt 2):150562
pubmed: 34852432
Parasit Vectors. 2021 Jan 6;14(1):11
pubmed: 33407818
Parasitol Res. 2018 Sep;117(9):2841-2846
pubmed: 29968038

Auteurs

Wei Zhao (W)

Department of Parasitology, Wenzhou Medical University, Wenzhou 325035, China - Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical University, Haikou 571199, China.

Yun Zhang (Y)

Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical University, Haikou 571199, China.

Jiaqi Li (J)

Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical University, Haikou 571199, China.

Guangxu Ren (G)

Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical University, Haikou 571199, China.

Yu Qiang (Y)

Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical University, Haikou 571199, China.

Yuan Wang (Y)

Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical University, Haikou 571199, China.

Xiuyi Lai (X)

Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical University, Haikou 571199, China.

Sheng Lei (S)

Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical University, Haikou 571199, China.

Rui Liu (R)

Department of Infectious and Tropical Diseases, the Second Affiliated Hospital of Hainan Medical University, Haikou 570100, China.

Yuankun Chen (Y)

Department of Infectious and Tropical Diseases, the Second Affiliated Hospital of Hainan Medical University, Haikou 570100, China.

Huicong Huang (H)

Department of Parasitology, Wenzhou Medical University, Wenzhou 325035, China.

Wenting Li (W)

Department of Infectious and Tropical Diseases, the Second Affiliated Hospital of Hainan Medical University, Haikou 570100, China.

Gang Lu (G)

Key Laboratory of Tropical Translational Medicine of Ministry of Education, NHC Key Laboratory of Tropical Disease Control, Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Department of Pathogenic Biology, Hainan Medical University, Haikou 571199, China - Department of Infectious and Tropical Diseases, the Second Affiliated Hospital of Hainan Medical University, Haikou 570100, China.

Feng Tan (F)

Department of Parasitology, Wenzhou Medical University, Wenzhou 325035, China.

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