Development of a plasma-free amino acid-based risk score for the incidence of cardiovascular diseases in a general population: The Nagahama study.
Amino acid
Cardiovascular disease
Coronary heart disease
General population
Risk score
Stroke
Journal
Clinical nutrition (Edinburgh, Scotland)
ISSN: 1532-1983
Titre abrégé: Clin Nutr
Pays: England
ID NLM: 8309603
Informations de publication
Date de publication:
Dec 2023
Dec 2023
Historique:
received:
03
04
2023
revised:
02
10
2023
accepted:
23
10
2023
medline:
27
11
2023
pubmed:
6
11
2023
entrez:
3
11
2023
Statut:
ppublish
Résumé
Levels of circulating amino acids (AAs) have been suggested to be associated with cardiovascular diseases (CVDs). This study aimed to develop a plasma-free amino acid (PFAA)-based CVD risk-prediction model in a general population. The study participants consisted of 9220 community residents (mean age, 53.2 years; standard deviation, 13.3 years). Circulating levels of 19 PFAAs were measured via high-performance liquid chromatography/electrospray ionization mass spectrometry. The incidence of CVDs was determined by reviewing participants' clinical records. The prediction model was developed using the Cox proportional hazards model with the brute force variable selection and then cross-validated. During the 8.5-year follow-up, 220 CVD events were observed. Six AAs (alanine, citrulline, glycine, histidine, serine, and tyrosine) were identified as components of the prediction model, of which the C-index was 0.72. The association between the fourth quartile of the risk score calculated using the prediction model and the CVD events was independent of conventional risk factors (adjusted hazard ratio [HR], 1.9; 95 % confidence interval, 1.1-3.3). When examining crude relationships between conventional risk factors and the PFAA-based risk score by subgroup analyses, the association was significant for most subpopulations, men [crude HR = 6.4 (2.0-20.2)] and women [crude HR = 4.9 (2.6-9.3)], and individuals with [crude HR = 4.7 (2.5-8.9)] and without [crude HR = 7.2 (2.7-18.9)] lifestyle-related diseases, but not for older (≥70 years) participants [crude HR = 3.3 (0.8-13.5)]. The risk score successfully identified at-risk individuals [HR = 2.1 (1.2-3.5)] from participants who were classified as low risk by a conventional CVD risk score. The PFAA-based risk score predicted CVD events independently of conventional risk factors.
Sections du résumé
BACKGROUND & AIMS
OBJECTIVE
Levels of circulating amino acids (AAs) have been suggested to be associated with cardiovascular diseases (CVDs). This study aimed to develop a plasma-free amino acid (PFAA)-based CVD risk-prediction model in a general population.
METHODS
METHODS
The study participants consisted of 9220 community residents (mean age, 53.2 years; standard deviation, 13.3 years). Circulating levels of 19 PFAAs were measured via high-performance liquid chromatography/electrospray ionization mass spectrometry. The incidence of CVDs was determined by reviewing participants' clinical records. The prediction model was developed using the Cox proportional hazards model with the brute force variable selection and then cross-validated.
RESULTS
RESULTS
During the 8.5-year follow-up, 220 CVD events were observed. Six AAs (alanine, citrulline, glycine, histidine, serine, and tyrosine) were identified as components of the prediction model, of which the C-index was 0.72. The association between the fourth quartile of the risk score calculated using the prediction model and the CVD events was independent of conventional risk factors (adjusted hazard ratio [HR], 1.9; 95 % confidence interval, 1.1-3.3). When examining crude relationships between conventional risk factors and the PFAA-based risk score by subgroup analyses, the association was significant for most subpopulations, men [crude HR = 6.4 (2.0-20.2)] and women [crude HR = 4.9 (2.6-9.3)], and individuals with [crude HR = 4.7 (2.5-8.9)] and without [crude HR = 7.2 (2.7-18.9)] lifestyle-related diseases, but not for older (≥70 years) participants [crude HR = 3.3 (0.8-13.5)]. The risk score successfully identified at-risk individuals [HR = 2.1 (1.2-3.5)] from participants who were classified as low risk by a conventional CVD risk score.
CONCLUSIONS
CONCLUSIONS
The PFAA-based risk score predicted CVD events independently of conventional risk factors.
Identifiants
pubmed: 37922695
pii: S0261-5614(23)00353-9
doi: 10.1016/j.clnu.2023.10.024
pii:
doi:
Substances chimiques
Citrulline
29VT07BGDA
Glycine
TE7660XO1C
Amines
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2512-2519Investigateurs
Yasuharu Tabara
(Y)
Takahisa Kawaguchi
(T)
Kazuya Setoh
(K)
Yoshimitsu Takahashi
(Y)
Shinji Kosugi
(S)
Takeo Nakayama
(T)
Fumihiko Matsuda
(F)
Informations de copyright
Copyright © 2023 The Author(s). Published by Elsevier Ltd.. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest Plasma amino acid levels were measured by Ajinomoto Co., Inc. The Center for Genomic Medicine received research funding, and YT and FM received consultation fees from Ajinomoto Co., Inc. MT, HY, KN, AI, and YK are employees of Ajinomoto Co., Inc. This does not alter the authors’ adherence to all of the journal policies. No other potential conflicts of interest in relation to this article are declared.