Detection of macrolide and fluoroquinolone resistance-associated 23S rRNA and parC mutations in Mycoplasma genitalium by nested real-time PCR.
Humans
Fluoroquinolones
/ pharmacology
Mycoplasma genitalium
/ genetics
RNA, Ribosomal, 23S
/ genetics
Real-Time Polymerase Chain Reaction
Macrolides
/ pharmacology
Microbial Sensitivity Tests
Drug Resistance, Bacterial
/ genetics
Mycoplasma Infections
/ diagnosis
Mycobacterium tuberculosis
/ genetics
Anti-Bacterial Agents
/ pharmacology
Mutation
23S rRNA
Mycoplasma genitalium
fluroquinolone
macrolide
nested real-time PCR
parC
Journal
Frontiers in cellular and infection microbiology
ISSN: 2235-2988
Titre abrégé: Front Cell Infect Microbiol
Pays: Switzerland
ID NLM: 101585359
Informations de publication
Date de publication:
2023
2023
Historique:
received:
02
08
2023
accepted:
10
10
2023
medline:
7
11
2023
pubmed:
6
11
2023
entrez:
6
11
2023
Statut:
epublish
Résumé
Traditional drug susceptibility testing cannot be performed in clinical laboratories due to the slow-growing characteristics of Sequencing analysis can detect unknown mutations, but it is time-consuming, requires professional analytical skills and the appropriate testing equipment. The main objective of this study was to establish a nested real-time PCR method for the simultaneous detection of 105 MG-positive samples and 27 samples containing other pathogens were used for validation. The limit of the nested real-time PCR detection was 500 copies/reaction and there was no cross-reaction with The results of this sensitive and rapid alternative for identifying resistant genotypes of
Sections du résumé
Background
Traditional drug susceptibility testing cannot be performed in clinical laboratories due to the slow-growing characteristics of
Objective
Sequencing analysis can detect unknown mutations, but it is time-consuming, requires professional analytical skills and the appropriate testing equipment. The main objective of this study was to establish a nested real-time PCR method for the simultaneous detection of
Results
105 MG-positive samples and 27 samples containing other pathogens were used for validation. The limit of the nested real-time PCR detection was 500 copies/reaction and there was no cross-reaction with
Conclusions
The results of this sensitive and rapid alternative for identifying resistant genotypes of
Identifiants
pubmed: 37928183
doi: 10.3389/fcimb.2023.1271392
pmc: PMC10623348
doi:
Substances chimiques
Fluoroquinolones
0
RNA, Ribosomal, 23S
0
Macrolides
0
Anti-Bacterial Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1271392Informations de copyright
Copyright © 2023 He, Yuan, Liang, Fan, Li and Pan.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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