Functional characterization of a naphthalene-O-methyltransferase from Nocardia sp. CS682.

Methyltransferases / chemistry Nocardia / metabolism Methylation S-Adenosylmethionine / metabolism Escherichia coli / metabolism Naphthalenes / metabolism
Enzyme kinetics Naphthalene Nocardia O-methyltransferase

Journal

Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761

Informations de publication

Date de publication:
Jan 2024
Historique:
received: 18 07 2023
revised: 24 10 2023
accepted: 25 10 2023
medline: 20 11 2023
pubmed: 9 11 2023
entrez: 8 11 2023
Statut: ppublish

Résumé

Methylation plays important roles in biosynthesis, metabolism, signal transduction, detoxification, protein sorting and repair, and nucleic acid processing. Generally the methyltransferases transfer methyl groups in various natural products using S-adenosyl methionine (SAM) as a cofactor. In this study, we examined and functionally characterized ThnM3 (enzyme), by testing various substrates with different chemical structures. Among the tested substrates, 1,8-dihydroxynaphthalene was the best substrate for methylation. Whole-cell biotransformation was performed using the enzyme in engineered Escherichia coli to produce 8-methoxynaphthalene-1-ol, and 1,8-dimethoxynaphthalene derivatives of 1,8-dihydroxynaphthalene. The products were confirmed using high-performance liquid chromatography, mass spectrometry, and nuclear magnetic resonance spectroscopic analyses. Therefore, this study is the first to amplify, express the thnM3 (gene), and functionally characterize theThnM3, which exhibits the regioselective modifications of 1,8-dihydroxynaphthalene.

Identifiants

DOI: 10.1016/j.enzmictec.2023.110351 PMID: 37939423
pubmed: 37939423
pii: S0141-0229(23)00159-X
doi: 10.1016/j.enzmictec.2023.110351
pii:
doi:

Substances chimiques

Methyltransferases EC 2.1.1.-
1,8-dihydroxynaphthalene 569-42-6
S-Adenosylmethionine 7LP2MPO46S
Naphthalenes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

110351

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no conflict of interest.

Auteurs

Purna Bahadur Poudel (PB)

Institute of Biomolecule Reconstruction (iBR), Department of Life Science and Biochemical Engineering, Sun Moon University, 70 Sun Moon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, South Korea.

Dipesh Dhakal (D)

Institute of Biomolecule Reconstruction (iBR), Department of Life Science and Biochemical Engineering, Sun Moon University, 70 Sun Moon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, South Korea.

Jong Cheol Lee (JC)

Department of Pharmaceutical Engineering and Biotechnology, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, South Korea.

Jae Kyung Sohng (JK)

Institute of Biomolecule Reconstruction (iBR), Department of Life Science and Biochemical Engineering, Sun Moon University, 70 Sun Moon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, South Korea; Department of Pharmaceutical Engineering and Biotechnology, Sun Moon University, 70 Sunmoon-ro 221, Tangjeong-myeon, Asan-si, Chungnam 31460, South Korea. Electronic address: sohng@sunmoon.ac.kr.

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Classifications MeSH

questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › One-carbon group transferases › Methyltransferases › Functional characterization of a...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Nocardiaceae › Nocardia › Functional characterization of a...
questionsmedicales.fr › Métabolisme › Alkylation › Méthylation › Functional characterization of a...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléosides › Ribonucléosides › Adénosine › Adémétionine › Functional characterization of a...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Functional characterization of a...
questionsmedicales.fr › Composés polycycliques › Hydrocarbures aromatiques polycycliques › Naphtalènes › Functional characterization of a...