In Vitro Imaging and Molecular Characterization of Ca
calcium
cell electrosensitization
electroporation
fractionated electric pulse protocol
mesenchymal stem cell
nanosecond pulsed electric fields
neuroblastoma cell
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
26 Oct 2023
26 Oct 2023
Historique:
received:
06
09
2023
revised:
18
10
2023
accepted:
23
10
2023
medline:
15
11
2023
pubmed:
14
11
2023
entrez:
14
11
2023
Statut:
epublish
Résumé
In recent years, the application of pulsed electric fields with very short durations (nanoseconds) and extremely high amplitudes (MV/m) has been investigated for novel medical purposes. Various electric protocols have been explored for different objectives, including the utilization of fractionated pulse doses to enhance cell electrosensitization to the uptake of different markers or an increase in apoptosis. This study focused on the use of fluorescence imaging to examine molecular calcium fluxes induced by different fractionated protocols of short electric pulses in neuroblastoma (SH-SY5Y) and mesenchymal stem cells (HaMSCs) that were electroporated using nanosecond pulsed electric fields. In our experimental setup, we did not observe cell electrosensitization in terms of an increase in calcium flux following the administration of fractionated doses of nanosecond pulsed electric fields with respect to the non-fractionated dose. However, we observed the targeted activation of calcium-dependent genes (
Identifiants
pubmed: 37958601
pii: ijms242115616
doi: 10.3390/ijms242115616
pmc: PMC10647260
pii:
doi:
Substances chimiques
Calcium
SY7Q814VUP
Calcium, Dietary
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : COST Action BM1309 European network for innovative uses of EMFs in biomedical applications (EMF-MED) Short Term Scientific Mission
ID : 1309
Organisme : Project RomeTechnopole, PNRR Mission 4 Component 2 Investment 1.5, funded by European Union - NextGenerationEU.
ID : ECS 0000024, - CUP. B83C22002820006
Références
Expert Rev Cardiovasc Ther. 2018 May;16(5):349-360
pubmed: 29595355
Int J Radiat Oncol Biol Phys. 2021 Apr 1;109(5):1495-1507
pubmed: 33509660
Proc Natl Acad Sci U S A. 2001 Sep 25;98(20):11024-31
pubmed: 11572963
Methods Mol Biol. 2008;423:351-9
pubmed: 18370213
Ann Biomed Eng. 2005 Feb;33(2):223-31
pubmed: 15771276
Nat Rev Mol Cell Biol. 2000 Oct;1(1):11-21
pubmed: 11413485
Bioact Mater. 2023 Apr 05;26:478-489
pubmed: 37090028
Biochem Soc Trans. 2001 May;29(Pt 2):350-3
pubmed: 11356181
Sci Rep. 2016 Mar 18;6:23225
pubmed: 26987779
Annu Rev Neurosci. 2008;31:563-90
pubmed: 18558867
Bioelectrochemistry. 2022 Oct;147:108218
pubmed: 35933972
Cell Calcium. 2011 Sep;50(3):211-21
pubmed: 21459443
PLoS One. 2016 Jul 25;11(7):e0159434
pubmed: 27454174
Sci Rep. 2017 Sep 8;7(1):10992
pubmed: 28887559
Lab Chip. 2012 Nov 21;12(22):4709-15
pubmed: 23037002
Arch Biochem Biophys. 2012 Nov 1;527(1):55-64
pubmed: 22910297
Neurosci Res. 2011 Mar;69(3):175-86
pubmed: 21163309
Cold Spring Harb Perspect Biol. 2011 Oct 01;3(10):a004259
pubmed: 21730044
Mol Biol Cell. 2002 Dec;13(12):4279-95
pubmed: 12475952
Biochim Biophys Acta. 2013 Mar;1828(3):981-9
pubmed: 23220180
Neurochem Res. 2014 Jan;39(1):129-41
pubmed: 24248860
J Neurosci. 2002 Sep 15;22(18):7892-902
pubmed: 12223542
Sci Rep. 2016 Aug 26;6:32331
pubmed: 27561994
J Cell Physiol. 2006 Jan;206(1):229-37
pubmed: 16021633
J Cell Mol Med. 2013 Jan;17(1):154-9
pubmed: 23305510
Acta Oncol. 2022 Sep;61(9):1093-1104
pubmed: 36036195
Am J Physiol Cell Physiol. 2003 Jun;284(6):C1438-47
pubmed: 12529240
Int J Mol Sci. 2022 Mar 10;23(6):
pubmed: 35328420
Biochim Biophys Acta. 2013 Aug;1830(8):4130-6
pubmed: 23583367
Int J Radiat Biol. 2022;98(1):109-121
pubmed: 34714724
Biochim Biophys Acta. 2009 Jun;1793(6):1096-104
pubmed: 19250948
PLoS One. 2011 Feb 09;6(2):e17100
pubmed: 21347394