Use of Localized Reconstruction to Visualize the Shigella Phage Sf6 Tail Apparatus.
Bacteriophages
Cryo-EM
Electron density
Localized reconstruction
Real-space refinement
Single-particle analysis
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2024
2024
Historique:
pmc-release:
01
01
2024
medline:
16
11
2023
pubmed:
15
11
2023
entrez:
15
11
2023
Statut:
ppublish
Résumé
Cryogenic electron microscopy (cryo-EM) single-particle analysis has revolutionized the structural analysis of icosahedral viruses, including tailed bacteriophages. In recent years, localized (or focused) reconstruction has emerged as a powerful data analysis method to capture symmetry mismatches and resolve asymmetric features in icosahedral viruses. Here, we describe the methods used to reconstruct the 2.65-MDa tail apparatus of the Shigella phage Sf6, a representative member of the Podoviridae superfamily.
Identifiants
pubmed: 37966602
doi: 10.1007/978-1-0716-3549-0_14
pmc: PMC10655839
mid: NIHMS1907213
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
215-228Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM100888
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM140733
Pays : United States
Organisme : NIH HHS
ID : S10 OD030457
Pays : United States
Organisme : NCI NIH HHS
ID : HHSN261200800001E
Pays : United States
Informations de copyright
© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
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