Origin of Discrete and Continuous Dark Noise in Rod Photoreceptors.
cGMP concentration
continuous noise
discrete noise
rod photoreceptor
thermal activation
visual pigment
Journal
eNeuro
ISSN: 2373-2822
Titre abrégé: eNeuro
Pays: United States
ID NLM: 101647362
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
received:
06
10
2023
accepted:
02
11
2023
medline:
1
12
2023
pubmed:
17
11
2023
entrez:
16
11
2023
Statut:
epublish
Résumé
The detection of a single photon by a rod photoreceptor is limited by two sources of physiological noise, called discrete and continuous noise. Discrete noise occurs as intermittent current deflections with a waveform very similar to that of the single-photon response to real light and is thought to be produced by spontaneous activation of rhodopsin. Continuous noise occurs as random and continuous fluctuations in outer-segment current and is usually attributed to some intermediate in the phototransduction cascade. To confirm the origin of these noise sources, we have recorded from retinas of mouse lines with rods having reduced levels of rhodopsin, transducin, or phosphodiesterase. We show that the rate of discrete noise is diminished in proportion to the decrease in rhodopsin concentration, and that continuous noise is independent of transducin concentration but clearly elevated when the level of phosphodiesterase is reduced. Our experiments provide new molecular evidence that discrete noise is indeed produced by rhodopsin itself, and that continuous noise is generated by spontaneous activation of phosphodiesterase resulting in random fluctuations in outer-segment current.
Identifiants
pubmed: 37973380
pii: ENEURO.0390-23.2023
doi: 10.1523/ENEURO.0390-23.2023
pmc: PMC10687842
pii:
doi:
Substances chimiques
Rhodopsin
9009-81-8
Transducin
EC 3.6.5.1
Phosphoric Diester Hydrolases
EC 3.1.4.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2023 Bocchero and Pahlberg.
Déclaration de conflit d'intérêts
The authors declare no competing financial interests.
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