FlgV forms a flagellar motor ring that is required for optimal motility of Helicobacter pylori.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2023
2023
Historique:
received:
28
03
2023
accepted:
07
06
2023
medline:
20
11
2023
pubmed:
17
11
2023
entrez:
17
11
2023
Statut:
epublish
Résumé
Flagella-driven motility is essential for Helicobacter pylori to colonize the human stomach, where it causes a variety of diseases, including chronic gastritis, peptic ulcer disease, and gastric cancer. H. pylori has evolved a high-torque-generating flagellar motor that possesses several accessories not found in the archetypical Escherichia coli motor. FlgV was one of the first flagellar accessory proteins identified in Campylobacter jejuni, but its structure and function remain poorly understood. Here, we confirm that deletion of flgV in H. pylori B128 and a highly motile variant of H. pylori G27 (G27M) results in reduced motility in soft agar medium. Comparative analyses of in-situ flagellar motor structures of wild-type, ΔflgV, and a strain expressing FlgV-YFP showed that FlgV forms a ring-like structure closely associated with the junction of two highly conserved flagellar components: the MS and C rings. The results of our studies suggest that the FlgV ring has adapted specifically in Campylobacterota to support the assembly and efficient function of the high-torque-generating motors.
Identifiants
pubmed: 37976320
doi: 10.1371/journal.pone.0287514
pii: PONE-D-23-09244
pmc: PMC10655999
doi:
Substances chimiques
Bacterial Proteins
0
Culture Media
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0287514Subventions
Organisme : NIAID NIH HHS
ID : R03 AI146907
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI140444
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI087946
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI132828
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI132818
Pays : United States
Informations de copyright
Copyright: © 2023 Botting et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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