Metabolic regulation boosts bioelectricity generation in Zymomonas mobilis microbial fuel cell, surpassing ethanol production.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
24 Nov 2023
Historique:
received: 15 07 2023
accepted: 19 11 2023
medline: 27 11 2023
pubmed: 25 11 2023
entrez: 24 11 2023
Statut: epublish

Résumé

Zymomonas mobilis (Z. mobilis), a bacterium known for its ethanol production capabilities, can also generate electricity by transitioning from ethanol production to electron generation. The purpose of this study is to investigate the ability of Z. mobilis to produce bioelectricity when utilized as a biocatalyst in a single-chamber microbial fuel cell (MFC). Given the bacterium's strong inclination towards ethanol production, a metabolic engineering strategy was devised to identify key reactions responsible for redirecting electrons from ethanol towards electricity generation. To evaluate the electroactivity of cultured Z. mobilis and its ethanol production in the presence of regulators, the reduction of soluble Fe(III) was utilized. Among the regulators tested, CuCl

Identifiants

pubmed: 38001147
doi: 10.1038/s41598-023-47846-7
pii: 10.1038/s41598-023-47846-7
pmc: PMC10673858
doi:

Substances chimiques

Ethanol 3K9958V90M
Ferric Compounds 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

20673

Informations de copyright

© 2023. The Author(s).

Références

Bioresour Technol. 2022 Mar;347:126579
pubmed: 34921921
Nat Protoc. 2011 Aug 04;6(9):1290-307
pubmed: 21886097
Nat Commun. 2018 Sep 7;9(1):3637
pubmed: 30194293
Microbiology (Reading). 2015 Dec;161(12):2384-94
pubmed: 26432557
Bioresour Technol. 2021 Jan;320(Pt B):124340
pubmed: 33189040
Nucleic Acids Res. 2019 Jan 8;47(D1):D542-D549
pubmed: 30395242
Mol Biosyst. 2016 Apr;12(4):1241-9
pubmed: 26883123
PLoS One. 2016 Apr 21;11(4):e0153866
pubmed: 27100889
Extremophiles. 2007 Mar;11(2):257-67
pubmed: 17072683
Bioresour Technol. 2017 Oct;242:101-108
pubmed: 28495054
Bioresour Technol. 2020 Mar 10;306:123173
pubmed: 32199399
Sci Rep. 2022 May 6;12(1):7417
pubmed: 35523838
Bioresour Technol. 2022 Feb;346:126588
pubmed: 34929329
Bioresour Technol. 2021 Dec;341:125772
pubmed: 34411941
Front Microbiol. 2019 Nov 14;10:2533
pubmed: 31798541
Biotechnol Prog. 2014 Sep-Oct;30(5):985-91
pubmed: 25044958
Biotechnol Adv. 2021 Dec;53:107682
pubmed: 33326817
Biotechnol Biofuels. 2020 Mar 05;13:36
pubmed: 32158500
Environ Sci Technol. 2010 Apr 1;44(7):2721-7
pubmed: 20199066
Biotechnol Biofuels. 2020 Jan 25;13:15
pubmed: 31998408
Trends Biotechnol. 2019 Sep;37(9):960-972
pubmed: 30876702
BMC Res Notes. 2021 May 28;14(1):208
pubmed: 34049566
Bioresour Technol. 2022 Sep;360:127581
pubmed: 35798169
Biotechnol Adv. 2018 Jul - Aug;36(4):1316-1327
pubmed: 29729376
Biosens Bioelectron. 2016 May 15;79:327-33
pubmed: 26720922
Microb Cell Fact. 2010 Nov 24;9:94
pubmed: 21092328
Biosens Bioelectron. 2012 Oct-Dec;38(1):264-9
pubmed: 22748963
Bioresour Technol. 2011 Feb;102(3):2758-66
pubmed: 21146983
Curr Opin Biotechnol. 2003 Oct;14(5):491-6
pubmed: 14580578
Bioresour Technol. 2021 Sep;336:125308
pubmed: 34044244
ACS Synth Biol. 2018 Mar 16;7(3):885-895
pubmed: 29429342
Metab Eng Commun. 2018 Nov 15;7:e00081
pubmed: 30591903
Sci Rep. 2021 Jun 10;11(1):12294
pubmed: 34112928
J Bacteriol. 2018 Sep 10;200(19):
pubmed: 30038047
Metab Eng. 2020 Sep;61:261-274
pubmed: 32590077

Auteurs

Hananeh Ahmadpanah (H)

Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115‑143, Tehran, Iran.

Ehsan Motamedian (E)

Department of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115‑143, Tehran, Iran. motamedian@modares.ac.ir.

Mohammad Mahdi Mardanpour (MM)

Department of Bioengineering, Faculty of Engineering, McGill University, Montreal, Canada.

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Classifications MeSH