ASCL1 induces neurogenesis in human Müller glia.
Journal
Stem cell reports
ISSN: 2213-6711
Titre abrégé: Stem Cell Reports
Pays: United States
ID NLM: 101611300
Informations de publication
Date de publication:
12 Dec 2023
12 Dec 2023
Historique:
received:
22
03
2023
revised:
27
10
2023
accepted:
30
10
2023
pubmed:
2
12
2023
medline:
2
12
2023
entrez:
1
12
2023
Statut:
ppublish
Résumé
In mammals, loss of retinal cells due to disease or trauma is an irreversible process that can lead to blindness. Interestingly, regeneration of retinal neurons is a well established process in some non-mammalian vertebrates and is driven by the Müller glia (MG), which are able to re-enter the cell cycle and reprogram into neurogenic progenitors upon retinal injury or disease. Progress has been made to restore this mechanism in mammals to promote retinal regeneration: MG can be stimulated to generate new neurons in vivo in the adult mouse retina after the over-expression of the pro-neural transcription factor Ascl1. In this study, we applied the same strategy to reprogram human MG derived from fetal retina and retinal organoids into neurons. Combining single cell RNA sequencing, single cell ATAC sequencing, immunofluorescence, and electrophysiology we demonstrate that human MG can be reprogrammed into neurogenic cells in vitro.
Identifiants
pubmed: 38039971
pii: S2213-6711(23)00423-X
doi: 10.1016/j.stemcr.2023.10.021
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2400-2417Informations de copyright
Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests This research is funded in part by a sponsored research agreement with Tenpoint Therapeutics; T.A.R. is a co-founder and consultant. Some of the findings in this manuscript are part of a patent that has been submitted by the University of Washington: PCT/US23/65219.