Borrelia puertoricensis in opossums (Didelphis marsupialis) from Colombia.
Borrelia puertoricensis
Colombia
Opossums
Tick-borne spirochetes
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
04 Dec 2023
04 Dec 2023
Historique:
received:
06
07
2023
accepted:
16
10
2023
medline:
6
12
2023
pubmed:
5
12
2023
entrez:
4
12
2023
Statut:
epublish
Résumé
The genus Borrelia comprises pathogenic species of bacteria that pose a significant risk to public health. Borrelia spp. are emerging or reemerging infectious agents worldwide with complex transmission cycles, and many species use rodents as vertebrate reservoir hosts. Spirochetes morphologically compatible with Borrelia have been recurrently observed in opossums; however, there is currently a lack of genetic evidence confirming infection or supporting that these marsupials are hosts of Borrelia spirochetes. During 2017, 53 serum samples of Didelphis marsupialis from the municipality of Colosó (department of Sucre, Colombia) were collected and allocated in a serum bank. DNA extracted from the serum samples was submitted to a Borrelia genus-specific real-time PCR targeting the 16S rRNA gene. Positive samples were subsequently derived from semi-nested PCR protocols to obtain large fragments of the 16S rRNA and flaB genes. Obtained amplicons were subjected to Sanger sequencing. One positive sample was randomly selected for next-generation sequencing (NGS). Obtained reads were mapped to genomes of Borrelia spp. and sequences of two genes used in a multilocus sequence typing scheme retrieved for taxonomic assignment and phylogenetic analyses. Overall, 18.8% (10/53) of the samples were positive by qPCR. Of them, 80% (8/10) and 60% (6/10) were positive for the 16S rRNA and flaB genes after semi-nested PCRs, respectively. Bioinformatic analysis of one sample sequenced with NGS yielded 22 reads of genus Borrelia with different sizes. Two housekeeping genes, rplB and pyrG, were recovered. Nucleotide pairwise comparisons and phylogenetic analyses of 16S rRNA, flaB, rplB and pyrG genes showed that the Borrelia sp. found in opossums from Colosó corresponded to Borrelia puertoricensis. We describe the first molecular evidence to our knowledge of B. puertoricensis in Colombia, specifically in opossums, and the first detection of this spirochete in a vertebrate host since its isolation from Ornithodoros puertoricensis in Panama. This detection is also relevant because of the epidemiological importance of opossums as reservoirs of zoonotic diseases to humans.
Sections du résumé
BACKGROUND
BACKGROUND
The genus Borrelia comprises pathogenic species of bacteria that pose a significant risk to public health. Borrelia spp. are emerging or reemerging infectious agents worldwide with complex transmission cycles, and many species use rodents as vertebrate reservoir hosts. Spirochetes morphologically compatible with Borrelia have been recurrently observed in opossums; however, there is currently a lack of genetic evidence confirming infection or supporting that these marsupials are hosts of Borrelia spirochetes.
METHODS
METHODS
During 2017, 53 serum samples of Didelphis marsupialis from the municipality of Colosó (department of Sucre, Colombia) were collected and allocated in a serum bank. DNA extracted from the serum samples was submitted to a Borrelia genus-specific real-time PCR targeting the 16S rRNA gene. Positive samples were subsequently derived from semi-nested PCR protocols to obtain large fragments of the 16S rRNA and flaB genes. Obtained amplicons were subjected to Sanger sequencing. One positive sample was randomly selected for next-generation sequencing (NGS). Obtained reads were mapped to genomes of Borrelia spp. and sequences of two genes used in a multilocus sequence typing scheme retrieved for taxonomic assignment and phylogenetic analyses.
RESULTS
RESULTS
Overall, 18.8% (10/53) of the samples were positive by qPCR. Of them, 80% (8/10) and 60% (6/10) were positive for the 16S rRNA and flaB genes after semi-nested PCRs, respectively. Bioinformatic analysis of one sample sequenced with NGS yielded 22 reads of genus Borrelia with different sizes. Two housekeeping genes, rplB and pyrG, were recovered. Nucleotide pairwise comparisons and phylogenetic analyses of 16S rRNA, flaB, rplB and pyrG genes showed that the Borrelia sp. found in opossums from Colosó corresponded to Borrelia puertoricensis.
CONCLUSIONS
CONCLUSIONS
We describe the first molecular evidence to our knowledge of B. puertoricensis in Colombia, specifically in opossums, and the first detection of this spirochete in a vertebrate host since its isolation from Ornithodoros puertoricensis in Panama. This detection is also relevant because of the epidemiological importance of opossums as reservoirs of zoonotic diseases to humans.
Identifiants
pubmed: 38049822
doi: 10.1186/s13071-023-06016-4
pii: 10.1186/s13071-023-06016-4
pmc: PMC10694944
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
448Informations de copyright
© 2023. The Author(s).
Références
Parasit Vectors. 2022 Jun 8;15(1):196
pubmed: 35676728
Bioinformatics. 2015 May 15;31(10):1674-6
pubmed: 25609793
Bioinformatics. 2018 Sep 1;34(17):i884-i890
pubmed: 30423086
Med Vet Entomol. 2023 Jun;37(2):219-227
pubmed: 36346249
Nucleic Acids Res. 2021 Jul 2;49(W1):W293-W296
pubmed: 33885785
Proc Natl Acad Sci U S A. 2008 Jun 24;105(25):8730-5
pubmed: 18574151
Zoonoses Public Health. 2021 Feb;68(1):12-18
pubmed: 33226201
Nat Methods. 2012 Mar 04;9(4):357-9
pubmed: 22388286
Res Vet Sci. 2007 Oct;83(2):145-9
pubmed: 17222877
PLoS One. 2018 Dec 26;13(12):e0208432
pubmed: 30586413
Curr Issues Mol Biol. 2021;42:97-112
pubmed: 33289682
Emerg Infect Dis. 2011 May;17(5):883-5
pubmed: 21529402
Cancer Res. 2017 Nov 1;77(21):e23-e26
pubmed: 29092932
Emerg Infect Dis. 2013 Dec;19(12):1948-55
pubmed: 24274654
Vector Borne Zoonotic Dis. 2009 Feb;9(1):109-18
pubmed: 18945194
Rev Bras Parasitol Vet. 2020 Jun 26;29(2):e000120
pubmed: 32609236
Nucleic Acids Res. 2004 Jan 1;32(Database issue):D23-6
pubmed: 14681350
Ticks Tick Borne Dis. 2020 Mar;11(2):101335
pubmed: 31836459
Parasitol Res. 2021 Dec;120(12):4091-4111
pubmed: 33788021
Protein Sci. 2018 Jan;27(1):135-145
pubmed: 28884485
J Clin Microbiol. 2003 Dec;41(12):5557-62
pubmed: 14662940
Mol Biol Evol. 2015 Jan;32(1):268-74
pubmed: 25371430
Int J Syst Bacteriol. 1996 Oct;46(4):859-65
pubmed: 8863409
PLoS Negl Trop Dis. 2021 Aug 16;15(8):e0009642
pubmed: 34398885
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Nat Methods. 2017 Jun;14(6):587-589
pubmed: 28481363
Bioinformatics. 2014 Jul 15;30(14):2068-9
pubmed: 24642063
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712