Yeast diversity in open agave fermentations across Mexico.
Kazachstania humilis
agave fermentation
agave spirits
open fermentation
saccharomycetales
yeasts diversity
Journal
Yeast (Chichester, England)
ISSN: 1097-0061
Titre abrégé: Yeast
Pays: England
ID NLM: 8607637
Informations de publication
Date de publication:
06 Dec 2023
06 Dec 2023
Historique:
revised:
10
11
2023
received:
03
07
2023
accepted:
15
11
2023
pubmed:
6
12
2023
medline:
6
12
2023
entrez:
6
12
2023
Statut:
aheadofprint
Résumé
Yeasts are a diverse group of fungal microorganisms that are widely used to produce fermented foods and beverages. In Mexico, open fermentations are used to obtain spirits from agave plants. Despite the prevalence of this traditional practice throughout the country, yeasts have only been isolated and studied from a limited number of distilleries. To systematically describe the diversity of yeast species from open agave fermentations, here we generate the YMX-1.0 culture collection by isolating 4524 strains from 68 sites with diverse climatic, geographical, and biological contexts. We used MALDI-TOF mass spectrometry for taxonomic classification and validated a subset of the strains by ITS and D1/D2 sequencing, which also revealed two potential novel species of Saccharomycetales. Overall, the composition of yeast communities was weakly associated with local variables and types of climate, yet a core set of six species was consistently isolated from most producing regions. To explore the intraspecific variation of the yeasts from agave fermentations, we sequenced the genomes of four isolates of the nonconventional yeast Kazachstania humilis. The genomes of these four strains were substantially distinct from a European isolate of the same species, suggesting that they may belong to different populations. Our work contributes to the understanding and conservation of an open fermentation system of great cultural and economic importance, providing a valuable resource to study the biology and genetic diversity of microorganisms living at the interface of natural and human-associated environments.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Consejo Nacional de Humanidades, Ciencias y Tecnologías de México
ID : FORDECYT-PRONACES/103000/2020
Organisme : Consejo Nacional de Humanidades, Ciencias y Tecnologías de México
ID : CB-2016-01/284992
Organisme : Consejo Nacional de Humanidades, Ciencias y Tecnologías de México
ID : 862595
Organisme : Consejo Nacional de Humanidades, Ciencias y Tecnologías de México
ID : 4133922
Organisme : Fondo de Investigación y Desarrollo Tecnológico del Cinvestav
ID : SEP-CINVESTAV/023
Organisme : Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica DGAPA-UNAM
ID : IN209021
Organisme : Programa de Apoyo a Proyectos de Investigación e Innovación Tecnológica DGAPA-UNAM
ID : IN230420
Organisme : UK Biotechnology and Biological Sciences Research Council (BBSRC), Global Challenges Research Fund (GCRF), CABANA Innovation Fund
ID : BB/P027849/1
Informations de copyright
© 2023 The Authors. Yeast published by John Wiley & Sons Ltd.
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