Mitochondria isolated from lipid droplets of white adipose tissue reveal functional differences based on lipid droplet size.
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
Feb 2024
Feb 2024
Historique:
received:
19
01
2023
revised:
20
10
2023
accepted:
23
10
2023
medline:
11
12
2023
pubmed:
7
12
2023
entrez:
6
12
2023
Statut:
epublish
Résumé
Recent studies in brown adipose tissue (BAT) described a unique subpopulation of mitochondria bound to lipid droplets (LDs), which were termed PeriDroplet Mitochondria (PDM). PDM can be isolated from BAT by differential centrifugation and salt washes. Contrary to BAT, this approach has so far not led to the successful isolation of PDM from white adipose tissue (WAT). Here, we developed a method to isolate PDM from WAT with high yield and purity by an optimized proteolytic treatment that preserves the respiratory function of mitochondria. Using this approach, we show that, contrary to BAT, WAT PDM have lower respiratory and ATP synthesis capacities compared with WAT cytoplasmic mitochondria (CM). Furthermore, by isolating PDM from LDs of different sizes, we found a negative correlation between LD size and the respiratory capacity of their PDM in WAT. Thus, our new isolation method reveals tissue-specific characteristics of PDM and establishes the existence of heterogeneity in PDM function determined by LD size.
Identifiants
pubmed: 38056907
pii: 7/2/e202301934
doi: 10.26508/lsa.202301934
pmc: PMC10700548
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NCI NIH HHS
ID : R01 CA232056
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK099618
Pays : United States
Organisme : NIAAA NIH HHS
ID : R01 AA026914
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG060456
Pays : United States
Organisme : NIA NIH HHS
ID : R21 AG063373
Pays : United States
Informations de copyright
© 2023 Brownstein et al.
Références
Cell Metab. 2018 Apr 3;27(4):869-885.e6
pubmed: 29617645
Int J Mol Sci. 2019 Oct 04;20(19):
pubmed: 31590292
J Mol Cell Cardiol. 2014 Mar;68:79-88
pubmed: 24434643
Dev Cell. 2015 Mar 23;32(6):678-92
pubmed: 25752962
Biochim Biophys Acta. 2013 May;1831(5):924-33
pubmed: 23376222
Biochemistry. 1964 Jun;3:783-91
pubmed: 14211616
J Lipid Res. 2011 Dec;52(12):2159-2168
pubmed: 21885430
EMBO Rep. 2018 Jan;19(1):57-72
pubmed: 29146766
J Cell Sci. 2017 Jan 15;130(2):315-324
pubmed: 28049719
Biochim Biophys Acta Mol Cell Biol Lipids. 2021 Oct;1866(10):158992
pubmed: 34147658
Cell Metab. 2009 Oct;10(4):324-35
pubmed: 19808025
Proc Nutr Soc. 2001 Aug;60(3):319-28
pubmed: 11681806
J Cell Biol. 1970 Aug;46(2):326-41
pubmed: 5449178
Diabetes. 2006 Dec;55(12):3418-28
pubmed: 17130488
Proc Natl Acad Sci U S A. 2003 Mar 18;100(6):3077-82
pubmed: 12629214
Acta Physiol (Oxf). 2019 Feb;225(2):e13182
pubmed: 30168663
Hepatology. 2015 Mar;61(3):870-82
pubmed: 25179419
J Pharmacol Toxicol Methods. 2018 May - Jun;91:50-58
pubmed: 29378341
EMBO J. 2017 Jun 1;36(11):1543-1558
pubmed: 28348166
J Cell Sci. 2012 Sep 1;125(Pt 17):4067-76
pubmed: 22685330
STAR Protoc. 2021 Aug 12;2(3):100735
pubmed: 34430910
Redox Biol. 2021 Oct;46:102087
pubmed: 34411987
Mol Cell Biol. 2003 Feb;23(3):1085-94
pubmed: 12529412
FASEB J. 2014 Oct;28(10):4408-19
pubmed: 25005176
J Biol Chem. 2009 Feb 20;284(8):5352-61
pubmed: 19049983
Protein Cell. 2019 Dec;10(12):921-926
pubmed: 31667701
Cancer Cell. 2015 Jun 8;27(6):864-76
pubmed: 26058080
J Atheroscler Thromb. 2017 Oct 1;24(10):989-998
pubmed: 28883211
Dev Cell. 2013 Feb 25;24(4):384-99
pubmed: 23415954
Exp Physiol. 2014 Sep;99(9):1168-78
pubmed: 25128326
Sci Rep. 2016 Dec 06;6:38310
pubmed: 27922115
Nat Rev Mol Cell Biol. 2019 Mar;20(3):137-155
pubmed: 30523332
Am J Pathol. 2009 Sep;175(3):927-39
pubmed: 19700756
J Cell Mol Med. 2016 May;20(5):794-803
pubmed: 26915330
Nature. 2023 Jan;613(7942):160-168
pubmed: 36477540
Cell Rep. 2023 May 30;42(5):112435
pubmed: 37104088
Physiol Rev. 2004 Jan;84(1):277-359
pubmed: 14715917
PLoS One. 2017 Aug 9;12(8):e0181046
pubmed: 28793320
Biochem J. 1988 Sep 15;254(3):677-83
pubmed: 3196285
Biochim Biophys Acta. 2015 May;1853(5):918-28
pubmed: 25655664
Elife. 2015 Nov 02;4:
pubmed: 26523392
Cells. 2019 Oct 11;8(10):
pubmed: 31614673
J Biol Chem. 2012 Jul 6;287(28):23852-63
pubmed: 22532565
Mol Cell. 2019 Dec 5;76(5):811-825.e14
pubmed: 31628041
Dev Cell. 2017 Jul 10;42(1):9-21.e5
pubmed: 28697336
J Cell Biol. 2019 Apr 1;218(4):1319-1334
pubmed: 30808705
Curr Protoc Cell Biol. 2016 Sep 01;72:3.15.1-3.15.13
pubmed: 27580706
Sci Rep. 2016 Jul 08;6:29539
pubmed: 27386790
Front Physiol. 2017 Jun 30;8:460
pubmed: 28713289
Sci Rep. 2017 Feb 20;7:42574
pubmed: 28218306
Front Cell Dev Biol. 2021 Sep 14;9:726261
pubmed: 34595176
Semin Cell Dev Biol. 2020 Dec;108:55-64
pubmed: 32446655
Bioinformatics. 2011 Apr 15;27(8):1179-80
pubmed: 21349861
J Biol Chem. 2008 Feb 8;283(6):3409-3417
pubmed: 18070881
Biochem Biophys Rep. 2016 Jul 1;6:101-107
pubmed: 27092336
Curr Biol. 2013 Aug 5;23(15):1489-96
pubmed: 23871243
STAR Protoc. 2020 Dec 30;2(1):100243
pubmed: 33458705
Biochemistry. 1965 May;4:897-901
pubmed: 14337707
J Proteome Res. 2012 Feb 3;11(2):1264-73
pubmed: 22268682
J Biol Chem. 2005 Dec 23;280(51):42325-35
pubmed: 16207721
Eat Weight Disord. 2000 Sep;5(3):132-42
pubmed: 11082792