Intradermal Vaccination with PLGA Nanoparticles via Dissolving Microneedles and Classical Injection Needles.
dissolving microneedle
intradermal drug delivery
nanoparticles
vaccine formulation
vaccine immunogenicity
Journal
Pharmaceutical research
ISSN: 1573-904X
Titre abrégé: Pharm Res
Pays: United States
ID NLM: 8406521
Informations de publication
Date de publication:
Feb 2024
Feb 2024
Historique:
received:
22
06
2023
accepted:
19
01
2024
medline:
21
2
2024
pubmed:
9
2
2024
entrez:
9
2
2024
Statut:
ppublish
Résumé
A dissolving microneedle array (dMNA) is a vaccine delivery device with several advantages over conventional needles. By incorporating particulate adjuvants in the form of poly(D,L-lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) into the dMNA, the immune response against the antigen might be enhanced. This study aimed to prepare PLGA-NP-loaded dMNA and to compare T-cell responses induced by either intradermally injected aqueous-PLGA-NP formulation or PLGA-NP-loaded dMNA in mice. PLGA NPs were prepared with microfluidics, and their physicochemical characteristics with regard to encapsulation efficiencies of ovalbumin (OVA) and CpG oligonucleotide (CpG), zeta potentials, polydispersity indexes, and sizes were analysed. PLGA NPs incorporated dMNA was produced with three different dMNA formulations by using the centrifugation method, and the integrity of PLGA NPs in dMNAs was evaluated. The immunogenicity was evaluated in mice by comparing the T-cell responses induced by dMNA and aqueous formulations containing ovalbumin and CpG (OVA/CpG) with and without PLGA NP. Prepared PLGA NPs had a size of around 100 nm. The dMNA formulations affected the particle integrity, and the dMNA with poly(vinyl alcohol) (PVA) showed almost no aggregation of PLGA NPs. The PLGA:PVA weight ratio of 1:9 resulted in 100% of penetration efficiency and the fastest dissolution in ex-vivo human skin (< 30 min). The aqueous formulation with soluble OVA/CpG and the aqueous-PLGA-NP formulation with OVA/CpG induced the highest CD4 + T-cell responses in blood and spleen cells. PLGA NPs incorporated dMNA was successfully fabricated and the aqueous formulation containing PLGA NPs induce superior CD4
Identifiants
pubmed: 38332390
doi: 10.1007/s11095-024-03665-7
pii: 10.1007/s11095-024-03665-7
pmc: PMC10879229
doi:
Substances chimiques
Polylactic Acid-Polyglycolic Acid Copolymer
1SIA8062RS
Ovalbumin
9006-59-1
Antigens
0
Lactic Acid
33X04XA5AT
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
305-319Subventions
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
ID : TTW15240
Informations de copyright
© 2024. The Author(s).
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