Biomolecular condensation orchestrates clathrin-mediated endocytosis in plants.

Endocytosis Cell Membrane / metabolism Clathrin / metabolism

Journal

Nature cell biology
ISSN: 1476-4679
Titre abrégé: Nat Cell Biol
Pays: England
ID NLM: 100890575

Informations de publication

Date de publication:
Mar 2024
Historique:
received: 24 04 2023
accepted: 10 01 2024
pmc-release: 01 09 2024
medline: 18 3 2024
pubmed: 13 2 2024
entrez: 12 2 2024
Statut: ppublish

Résumé

Clathrin-mediated endocytosis is an essential cellular internalization pathway involving the dynamic assembly of clathrin and accessory proteins to form membrane-bound vesicles. The evolutionarily ancient TSET-TPLATE complex (TPC) plays an essential, but ill-defined role in endocytosis in plants. Here we show that two highly disordered TPC subunits, AtEH1 and AtEH2, function as scaffolds to drive biomolecular condensation of the complex. These condensates specifically nucleate on the plasma membrane through interactions with anionic phospholipids, and facilitate the dynamic recruitment and assembly of clathrin, as well as early- and late-stage endocytic accessory proteins. Importantly, condensation promotes ordered clathrin assemblies. TPC-driven biomolecular condensation thereby facilitates dynamic protein assemblies throughout clathrin-mediated endocytosis. Furthermore, we show that a disordered region of AtEH1 controls the material properties of endocytic condensates in vivo. Alteration of these material properties disturbs the recruitment of accessory proteins, influences endocytosis dynamics and impairs plant responsiveness. Our findings reveal how collective interactions shape endocytosis.

Identifiants

DOI: 10.1038/s41556-024-01354-6 PMID: 38347182 PMC: PMC7615741
pubmed: 38347182
doi: 10.1038/s41556-024-01354-6
pii: 10.1038/s41556-024-01354-6
pmc: PMC7615741
mid: EMS194255
doi:

Substances chimiques

Clathrin 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

438-449

Subventions

Organisme : European Research Council
ID : 682436
Pays : International
Organisme : European Research Council
ID : 803048
Pays : International
Organisme : European Research Council
ID : 852136
Pays : International

Informations de copyright

© 2024. The Author(s), under exclusive licence to Springer Nature Limited.

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Auteurs

Jonathan Michael Dragwidge (JM)

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium. jodra@psb.vib-ugent.be.
VIB Center for Plant Systems Biology, Ghent, Belgium. jodra@psb.vib-ugent.be.
ORCID: 0000-0002-3770-8374

Yanning Wang (Y)

Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.

Lysiane Brocard (L)

Bordeaux Imaging Center, INSERM, CNRS, Université de Bordeaux, Bordeaux, France.

Andreas De Meyer (A)

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Roman Hudeček (R)

Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic.
ORCID: 0000-0002-2271-7104

Dominique Eeckhout (D)

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Peter Grones (P)

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Matthieu Buridan (M)

Bordeaux Imaging Center, INSERM, CNRS, Université de Bordeaux, Bordeaux, France.

Clément Chambaud (C)

Laboratoire de Biogenèse Membranaire, CNRS, Université de Bordeaux, Bordeaux, France.

Přemysl Pejchar (P)

Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic.

Martin Potocký (M)

Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic.

Joanna Winkler (J)

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Michaël Vandorpe (M)

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Nelson Serre (N)

Department of Experimental Plant Biology, Faculty of Sciences, Charles University, Prague, Czech Republic.

Matyáš Fendrych (M)

Department of Experimental Plant Biology, Faculty of Sciences, Charles University, Prague, Czech Republic.

Amelie Bernard (A)

Laboratoire de Biogenèse Membranaire, CNRS, Université de Bordeaux, Bordeaux, France.

Geert De Jaeger (G)

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium.
VIB Center for Plant Systems Biology, Ghent, Belgium.

Roman Pleskot (R)

Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czech Republic.
ORCID: 0000-0003-0436-9748

Xiaofeng Fang (X)

Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.
ORCID: 0000-0003-1161-9186

Daniël Van Damme (D)

Department of Plant Biotechnology and Bioinformatics, Ghent University, Ghent, Belgium. dadam@psb.vib-ugent.be.
VIB Center for Plant Systems Biology, Ghent, Belgium. dadam@psb.vib-ugent.be.
ORCID: 0000-0002-9385-4851

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Endocytose › Biomolecular condensation orchestrates...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Biomolecular condensation orchestrates...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines du transport vésiculaire › Clathrine › Biomolecular condensation orchestrates...