Waste cooking oil and molasses for the sustainable production of extracellular lipase by Saitozyma flava.
circular economy
fermentation
lipase production
molasses
unconventional yeasts
waste cooking oil
Journal
Biotechnology and applied biochemistry
ISSN: 1470-8744
Titre abrégé: Biotechnol Appl Biochem
Pays: United States
ID NLM: 8609465
Informations de publication
Date de publication:
26 Feb 2024
26 Feb 2024
Historique:
received:
19
07
2023
accepted:
10
02
2024
pubmed:
27
2
2024
medline:
27
2
2024
entrez:
27
2
2024
Statut:
aheadofprint
Résumé
Organic waste valorization is one of the principal goals of the circular economy. Bioprocesses offer a promising approach to achieve this goal by employing microorganisms to convert organic feedstocks into high value products through their metabolic activities. In this study, a fermentation process for yeast cultivation and extracellular lipase production was developed by utilizing food waste. Lipases are versatile enzymes that can be applied in a wide range of industrial fields, from detergent, leather, and biodiesel production to food and beverage manufacturing. Among several oleaginous yeast species screened, Saitozyma flava was found to exhibit the highest secreted lipase activity on pNP-butyrate, pNP-caproate, and pNP-caprylate. The production medium was composed of molasses, a by-product of the sugar industry, which provided nutrients for yeast biomass formation. At the same time, waste cooking oil was employed to induce and enhance extracellular lipase production. After 48 h of process, 20 g/L of yeast biomass and 150 mU/mg
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Fondazione Cariplo
Organisme : Cariplo Foundation (Italy)
ID : ID 2020-1094
Organisme : Cariplo Foundation (Italy)
ID : BioSurf
Informations de copyright
© 2024 The Authors. Biotechnology and Applied Biochemistry published by Wiley Periodicals LLC on behalf of International Union of Biochemistry and Molecular Biology.
Références
Ollis DL, Cheah E, Cygler M, Dijkstra B, Frolow F, Franken SM, et al. The alpha/beta hydrolase fold. Protein Eng. 1992;5:197-211.
Lenfant N, Hotelier T, Velluet E, Bourne Y, Marchot P, Chatonnet A. ESTHER, the database of the a/b-hydrolase fold superfamily of proteins: tools to explore diversity of functions. Nucleic Acids Res. 2013;1:423-429.
Karadzic I, Masui A, Zivkovic LI, Fujiwara N. Purification and characterization of an alkaline lipase from Pseudomonas aeruginosa isolated from putrid mineral cutting oil as component of metalworking fluid. J Biosci Bioeng. 2006;102(2):82-89.
Ergan F, Trani M, Andre G. Production of glycerides from glycerol and fatty acid by immobilized lipases in non-aqueous media. Biotechnol Bioeng. 1990;35(2):195-200.
Carvalho AC, Fonseca TD, Mattos MC, Oliveira MD, Lemos TL, Molinari F, et al. Recent advances in lipase-mediated preparation of pharmaceuticals and their intermediates. Int J Mol Sci. 2015;16(12):29682-29716. https://doi.org/10.3390/ijms161226191
Rajendran A, Palanisamy A, Thangavelu V. Lipase catalyzed ester synthesis for food processing industries. Brazil Arch Biol Technol. 2009;52(1):207-219.
Chandra P, Enespa SR, Arora PK. Microbial lipases and their industrial applications: a comprehensive review. Microb Cell Fact. 2020;19:169 https://doi.org/10.1186/s12934-020-01428-8
Mendes AA, Oliveira PC, de Castro HF. Properties and biotechnological applications of porcine pancreatic lipase. J Mol Catal Enzym. 2012;78:119-134.
Reetz MT. Biocatalysis in organic chemistry and biotechnology: past, present, and future. J Am Chem Soci. 2013;135(34):12480-12496.
Elend C, Schmeisser C, Hoebenreich H, Steele HL, Streit WR. Isolation and characterization of a metagenome-derived and cold-active lipase with high stereospecificity for (R)-ibuprofen esters. J Biotechnol. 2007;130(4):370-377.
Sánchez-Porro C, Martin S, Mellado E, Ventosa A. Diversity of moderately halophilic bacteria producing extracellular hydrolytic enzymes. J Appl Microbiol. 2003;94(2):295-300.
Boutaiba S, Bhatnagar T, Hacene H, Mitchell DA, Baratti JC. Preliminary characterization of a lipolytic activity from an extremely halophilic archeon, Natronococcus sp. J Mol Catal B Enz. 2006;41:21-26
Mayordomo I, Randez-Gil F, Prieto JA. Isolation, purification, and characterization of a cold-active lipase from Aspergillus nidulans. J Agric Food Chem. 2000;48(1):105-109.
Girotto F, Alibardi L, Cossu R. Food waste generation and industrial uses: a review. Waste Manag. 2015;45:32-41.
Homrich A, Galvão G, Gamboa Abadia L, Carvalho MM. The circular economy umbrella: trends and gaps on integrating pathways. J Clean Prod. 2018;175:525-543.
Sarrouh B, Santos TM, Miyoshi A, Dias R, Azevedo V. Up-to-date insight on industrial enzymes applications and global market. J Bioprocess Biotechnol. 2012;S4:002. https://doi.org/10.4172/2155-9821.S4-002
Barbosa HS, de Silveira EA, Miranda M, Ernandes JR. Efficient very-high gravity fermentation of sugarcane molasses by industrial yeast strains. J Inst Brew. 2016;122:329-333. https://doi.org/10.1002/jib.317
Sánchez ÓJ, Cardona CA. Trends in biotechnological production of fuel ethanol from different feedstocks. Bioresour Technol. 2008;99:5270-5295.
Azhar SHM, Abdulla R, Jambo SA, Marbawi H, Gansau JA, Faik AAM, et al. Yeasts in sustainable bioethanol production: a review. Biochem Biophys Reports. 2017;10:52-61.
Ikram-ul H, Ali S, Qadeer MA, Iqbal J. Citric acid production by selected mutants of Aspergillus niger from cane molasses. Bioresour Technol. 2004;93:125-130. https://doi.org/10.1016/j.biortech.2003.10.018
Kotzamanidis C, Roukas T, Skaracis G. Optimization of lactic acid production from beet molasses by Lactobacillus delbrueckii NCIMB 8130. World J Microbiol Biotechnol. 2002;18:441-448. https://doi.org/10.1023/A:1015523126741
Ghazi I, Fernandez-Arrojo L, Gomez De Segura A, Alcalde M, Plou FJ, Ballesteros A. Beet sugar syrup and molasses as low-cost feedstock for the enzymatic production of fructo-oligosaccharides. J Agric Food Chem. 2006;54:2964-2968. https://doi.org/10.1021/jf053023b
Kamini NR, Fujii T, Kurosu T, Iefuji H. Production, purification and characterization of an extracellular lipase from the yeast, Cryptococcus sp. S-2. Process Biochem. 2000;36:317-324.
Ikemoto M, Ota Y. Production of two types of non-specific lipases by Geotrichum sp. FO 274A: a fish oil-assimilating strain. J Gen Appl Microbiol. 1996;42:371-379.
Global No. 1. Business data platform. Hamburg: Statista; 2023. Available from. https://www.statista.com/statistics/263937/vegetableoils-global-consumption (Accessed 13 June 2023)
Dizge N, Aydiner C, Imer DY, Bayramoglu M, Tanriseven A, Keskinler B. Biodiesel production from sunflower, soybean, and waste cooking oils by transesterification using lipase immobilized onto a novel microporous polymer. Bioresour Technol. 2009;100:1983-1991. https://doi.org/10.1016/j.biortech.2008.10.008
Yaakob Z, Mohammad M, Alherbawi M, Alam Z, Sopian K. Overview of the production of biodiesel from waste cooking oil. Renew Sustain Energy Rev. 2013;18:184-193. https://doi.org/10.1016/j.rser.2012.10.016
Mannu A, Garroni S, Ibanez Porras J, Mele A. Available technologies and materials for waste cooking oil recycling. Processes. 2020;8:366.
Liu Y, Zhang J, Li Q, Wang Z, Cui Z, Su T, et al. Engineering Yarrowia lipolytica for the sustainable production of β-farnesene from waste oil feedstock. Biotechnol Biofuels. 2022;15:101. https://doi.org/10.1186/s13068-022-02201-2
Zhao Y, Zhu K, Li J, Zhao Y, Li S, Zhang C, et al. High-efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica. Microb Biotechnol. 2021;14(6):2497-2513. https://doi.org/10.1111/1751-7915.13768
Nunes PMB, Fraga JL, Ratier RB, Rocha-Leão MHM, Brígida AIS, Fickers P, et al. Waste soybean frying oil for the production, extraction, and characterization of cell-wall-associated lipases from Yarrowia lipolytica. Bioprocess Biosyst Eng. 2021;44:809-818.
Lopes M, Miranda SM, Alves JM, Pereira AS, Belo, I. Waste cooking oils as feedstock for lipase and lipid-rich biomass production. Eur J Lipid Sci Technol. 2019;121(1):1800188. https://doi.org/10.1002/ejlt.201800188
Fickers P, Marty A, Nicaud JM. The lipases from Yarrowia lipolytica: genetics, production, regulation, biochemical characterization and biotechnological applications. Biotechnol Adv. 2011;29:632-644, https://doi.org/10.1016/j.biotechadv.2011.04.005
Potumarthi P, Subhakar C, Vanajakshi J, Jetty A. Effect of aeration and agitation regimes on lipase production by newly isolated Rhodotorula mucillaginosa-MTCC 8737 in stirred tank reactor using molasses as sole carbon source. Appl Biochem Biotechnol. 2008;151:700-710.
Lukaszewicz M, Jablonski S, Krasowska A. Characterization of alkaline lipase from an arctic yeast strain Rhodosporidium babjevae BD19. Eur Sci J ESJ. 2013;9:21:1480-1489
Yalçın HT, Çorbacı C, Uçar FB. Molecular characterization and lipase profiling of the yeasts isolated from environments contaminated with petroleum. J Basic Microbiol Suppl. 2013;1:S85-S92.
Donzella S, Cucchetti D, Capusoni C, Rizzi A, Galafassi S, Chiara G, et al. Engineering cytoplasmic acetyl-CoA synthesis decouples lipid production from nitrogen starvation in the oleaginous yeast Rhodosporidium azoricum. Microb Cell Fact. 2019;18:199.
Gupta N, Rathi P, Gupta R. Simplified para-nitrophenyl palmitate assay for lipases and esterases. Anal Biochem. 2002;311:98-99. https://doi.org/10.1016/S0003-2697(02)00379-2
Dalmau C, Montesinos JL, Lotti M, Casas C. Effect of different carbon sources on lipase production by Candida rugosa. Enzyme Microb Technol. 2000;26(9-10):657-663, https://doi.org/10.1016/S0141-0229(00)00156-3
Liu XZ, Wang QM, Göker M, Groenewald M, Kachalkin AV, Lumbsch HT, et al. Towards an integrated phylogenetic classification of the Tremellomycetes. Stud Mycol. 2015;81:85-147. https://doi.org/10.1016/j.simyco.2015.12.001
Fickers P, Nicaud JM, Gaillardin C, Destain J, Thonart P. Carbon and nitrogen sources modulate lipase production in the yeast Yarrowia lipolytica. J Appl Microbiol. 2004;96:742-774.
Turki S, Kraeim IB, Weeckers F, Thonart P, Kallel H. Isolation of bioactive peptides from tryptone that modulate lipase production in Yarrowia lipolytica. Bioresour Technol. 2009;100:2724-2731.
Ferrer P, Montesinos JL, Valero F, Sola C. Production of native and recombinant lipases by Candida rugosa: a review. Appl Biochem Biotechnol. 2001;95:221-255.
Fickers P, Fudalej F, Nicaud JM, Destain J, Thonart P. Selection of new over-producing derivatives for the improvement of extracellular lipase production by the nonconventional yeast Yarrowia lipolytica. J Biotechnol. 2005;115:379-386.
Xiaoyan L, Yu X, Lv J, Xu J, Xia J, Wu Z, et al. A cost-effective process for the coproduction of erythritol and lipase with Yarrowia lipolytica M53 from waste cooking oil. Food Bioprod Process, 2017;103:86-94.
Kuno H, Ota Y. The new method for the purification of extracellular lipases from Yarrowia (Saccharomycopsis) lipolytica and some properties of lipase A. J Fac Appl Biol Sci. 1996;35:191-197.
Pignède G, Wang H, Fudalej F, Gaillardin C, Seman M, Nicaud JM. Characterization of an extracellular lipase encoded by LIP2 in Yarrowia lipolytica. J Bacteriol. 2000;182(10):2802-2810. https://doi.org/10.1128/JB.182.10.2802-2810.2000
Brígida IS, Amaral FF, Gonçalves RB, da Rocha-Leão MHM, Coelho MAZ. Yarrowia lipolytica IMUFRJ 50682: lipase production in a multiphase bioreactor. Curr Biochem Eng. 2013;1:65-74. https://doi.org/10.2174/22127119113019990005
Carvalho T, Finotelli PV, Bonomo RCF, Franco M, Amaral PFF. Evaluating aqueous two-phase systems for Yarrowia lipolytica extracellular lipase purification. Process Biochem. 2017;53:259-266. https://doi.org/10.1016/j.procbio.2016.11.019
Donzella S, Fumagalli A, Arioli S, Pellegrino L, D'Incecco P, Molinari F, et al. Recycling food waste and saving water: optimization of the fermentation processes from cheese whey permeate to yeast oil. Fermentation. 2022;8(7):341. https://doi.org/10.3390/fermentation8070341
Najjar A, Robert S, Guérin C, Violet-Asther M, Carrière F. Quantitative study of lipase secretion, extracellular lipolysis, and lipid storage in the yeast Yarrowia lipolytica grown in the presence of olive oil: analogies with lipolysis in humans. Appl Microbiol Biotechnol. 2011;89:1947-1962. https://doi.org/10.1007/s00253-010-2993-5