Inhibition of leukocyte migration after ischemic stroke by VE-cadherin mutation in a mouse model leads to reduced infarct volumes and improved motor skills.
MCAO
VE-cadherin mutations
ischemic cascade
ischemic stroke
knock-in mice
leukocyte migration
vascular permeability
Journal
Brain and behavior
ISSN: 2162-3279
Titre abrégé: Brain Behav
Pays: United States
ID NLM: 101570837
Informations de publication
Date de publication:
Mar 2024
Mar 2024
Historique:
revised:
28
01
2024
received:
18
08
2023
accepted:
04
02
2024
medline:
13
3
2024
pubmed:
12
3
2024
entrez:
12
3
2024
Statut:
ppublish
Résumé
To distinguish between the genuine cellular impact of the ischemic cascade by leukocytes and unspecific effects of edema and humoral components, two knock-in mouse lines were utilized. Mouse lines Y731F and Y685F possess point mutations in VE-cadherin, which lead to a selective inhibition of transendothelial leukocyte migration or impaired vascular permeability. Ischemic stroke was induced by a model of middle cerebral artery occlusion. Analysis contained structural outcomes (infarct volume and extent of brain edema), functional outcomes (survival analysis, rotarod test, and neuroscore), and the extent and spatial distribution of leukocyte migration (heatmaps and fluorescence-activated cell sorting (FACS) analysis). Inhibition of transendothelial leukocyte migration as in Y731F mice leads to smaller infarct volumes (52.33 ± 4719 vs. 70.43 ± 6483 mm Selective inhibition of transendothelial leukocyte migration by VE-cadherin mutation after ischemic stroke in a mouse model leads to smaller infarct volumes and improved motor skills.
Identifiants
pubmed: 38468566
doi: 10.1002/brb3.3449
pmc: PMC10928452
doi:
Substances chimiques
cadherin 5
0
Antigens, CD
0
Cadherins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e3449Subventions
Organisme : Interdisciplinary Center for Clinical Research (IZKF) of the Medical Faculty of the University of Münster (SEED 07)
Informations de copyright
© 2024 The Authors. Brain and Behavior published by Wiley Periodicals LLC.
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