Triggered contraction of self-assembled micron-scale DNA nanotube rings.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
14 Mar 2024
14 Mar 2024
Historique:
received:
13
09
2023
accepted:
21
02
2024
medline:
18
3
2024
pubmed:
15
3
2024
entrez:
15
3
2024
Statut:
epublish
Résumé
Contractile rings are formed from cytoskeletal filaments during cell division. Ring formation is induced by specific crosslinkers, while contraction is typically associated with motor protein activity. Here, we engineer DNA nanotubes and peptide-functionalized starPEG constructs as synthetic crosslinkers to mimic this process. The crosslinker induces bundling of ten to hundred DNA nanotubes into closed micron-scale rings in a one-pot self-assembly process yielding several thousand rings per microliter. Molecular dynamics simulations reproduce the detailed architectural properties of the DNA rings observed in electron microscopy. Theory and simulations predict DNA ring contraction - without motor proteins - providing mechanistic insights into the parameter space relevant for efficient nanotube sliding. In agreement between simulation and experiment, we obtain ring contraction to less than half of the initial ring diameter. DNA-based contractile rings hold promise for an artificial division machinery or contractile muscle-like materials.
Identifiants
pubmed: 38485920
doi: 10.1038/s41467-024-46339-z
pii: 10.1038/s41467-024-46339-z
pmc: PMC10940629
doi:
Substances chimiques
Proteins
0
Myosins
EC 3.6.4.1
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2307Subventions
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : EXC-2082/1 - 390761711
Organisme : EC | EU Framework Programme for Research and Innovation H2020 | H2020 Priority Excellent Science | H2020 European Research Council (H2020 Excellent Science - European Research Council)
ID : 101076997
Informations de copyright
© 2024. The Author(s).
Références
Phys Rev Lett. 2007 Jul 27;99(4):048101
pubmed: 17678408
Phys Rev Lett. 2023 Jul 21;131(3):038401
pubmed: 37540881
Chemistry. 2015 Feb 16;21(8):3178-82
pubmed: 25581338
Nat Chem Biol. 2019 Aug;15(8):830-837
pubmed: 31320758
Nat Methods. 2012 Jun 28;9(7):676-82
pubmed: 22743772
J Am Chem Soc. 2022 Jun 8;144(22):10075-10079
pubmed: 35617523
ACS Synth Biol. 2022 Oct 21;11(10):3120-3133
pubmed: 36164967
Chem Sci. 2015 Apr 1;6(4):2252-2267
pubmed: 29308139
ACS Nano. 2022 May 24;16(5):7233-7241
pubmed: 35377150
Science. 2022 Mar 11;375(6585):1159-1164
pubmed: 35271337
Nanoscale. 2023 Feb 9;15(6):2904-2910
pubmed: 36691928
Elife. 2021 Jul 16;10:
pubmed: 34269679
J Am Chem Soc. 2004 Dec 22;126(50):16344-52
pubmed: 15600335
Biol Proced Online. 2009 May 15;11:32-51
pubmed: 19495910
Nano Lett. 2017 Dec 13;17(12):7283-7288
pubmed: 29182337
Nat Nanotechnol. 2015 Jun;10(6):528-33
pubmed: 25961509
Nano Lett. 2013 Sep 11;13(9):4006-13
pubmed: 23919535
Cell. 2015 Mar 12;160(6):1159-68
pubmed: 25748652
Nat Commun. 2021 Jul 26;12(1):4531
pubmed: 34312383
Phys Rev E Stat Nonlin Soft Matter Phys. 2002 Jun;65(6 Pt 1):061904
pubmed: 12188756
Small. 2020 Feb;16(6):e1905987
pubmed: 31917513
Nat Cell Biol. 2014 Apr;16(4):322-34
pubmed: 24633326
Nat Cell Biol. 2015 Apr;17(4):480-9
pubmed: 25799060
Mol Biol Cell. 2019 Nov 15;30(24):2953-2968
pubmed: 31599700
Nat Commun. 2024 Mar 14;15(1):2307
pubmed: 38485920
ACS Appl Mater Interfaces. 2018 May 2;10(17):14264-14270
pubmed: 29658265
Nat Chem. 2022 Aug;14(8):958-963
pubmed: 35725773
Nucleic Acids Res. 2011 Mar;39(4):1419-26
pubmed: 20952402
Biochemistry. 1986 Oct 21;25(21):6432-8
pubmed: 3790531
Phys Rev Lett. 2005 Jul 15;95(3):038102
pubmed: 16090774
Nano Lett. 2015 Feb 11;15(2):1368-73
pubmed: 25611357
Dev Cell. 2012 Jun 12;22(6):1247-60
pubmed: 22698284
Proc Natl Acad Sci U S A. 2021 Jul 6;118(27):
pubmed: 34187892
ACS Nano. 2013 Aug 27;7(8):6700-10
pubmed: 23879368
ACS Nano. 2021 Aug 24;15(8):12768-12779
pubmed: 34170119
J Am Chem Soc. 2013 Feb 27;135(8):2919-22
pubmed: 23388040
Curr Biol. 2011 Dec 20;21(24):R976-8
pubmed: 22192825
Biophys J. 2008 Sep 15;95(6):3059-65
pubmed: 18586848
Nat Commun. 2021 Jul 28;12(1):4595
pubmed: 34321459
Nat Chem. 2016 May;8(5):476-83
pubmed: 27102682
Nat Commun. 2021 Apr 15;12(1):2254
pubmed: 33859190