Metagenomic insights into the wastewater resistome before and after purification at large‑scale wastewater treatment plants in the Moscow city.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 03 2024
15 03 2024
Historique:
received:
26
10
2023
accepted:
12
03
2024
medline:
18
3
2024
pubmed:
16
3
2024
entrez:
16
3
2024
Statut:
epublish
Résumé
Wastewater treatment plants (WWTPs) are considered to be hotspots for the spread of antibiotic resistance genes (ARGs). We performed a metagenomic analysis of the raw wastewater, activated sludge and treated wastewater from two large WWTPs responsible for the treatment of urban wastewater in Moscow, Russia. In untreated wastewater, several hundred ARGs that could confer resistance to most commonly used classes of antibiotics were found. WWTPs employed a nitrification/denitrification or an anaerobic/anoxic/oxic process and enabled efficient removal of organic matter, nitrogen and phosphorus, as well as fecal microbiota. The resistome constituted about 0.05% of the whole metagenome, and after water treatment its share decreased by 3-4 times. The resistomes were dominated by ARGs encoding resistance to beta-lactams, macrolides, aminoglycosides, tetracyclines, quaternary ammonium compounds, and sulfonamides. ARGs for macrolides and tetracyclines were removed more efficiently than beta-lactamases, especially ampC, the most abundant ARG in the treated effluent. The removal efficiency of particular ARGs was impacted by the treatment technology. Metagenome-assembled genomes of multidrug-resistant strains were assembled both for the influent and the treated effluent. Ccomparison of resistomes from WWTPs in Moscow and around the world suggested that the abundance and content of ARGs depend on social, economic, medical, and environmental factors.
Identifiants
pubmed: 38491069
doi: 10.1038/s41598-024-56870-0
pii: 10.1038/s41598-024-56870-0
pmc: PMC10942971
doi:
Substances chimiques
Wastewater
0
Anti-Bacterial Agents
0
Tetracyclines
0
Macrolides
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6349Subventions
Organisme : Russian Science Foundation
ID : 22-74-00022
Informations de copyright
© 2024. The Author(s).
Références
Nat Biotechnol. 2018 Nov;36(10):996-1004
pubmed: 30148503
Microbiol Spectr. 2019 Jul;7(4):
pubmed: 31298205
Microbiology (Reading). 2009 Jul;155(Pt 7):2306-2319
pubmed: 19389756
Clin Microbiol Infect. 2012 Mar;18(3):268-81
pubmed: 21793988
Antimicrob Agents Chemother. 2005 Feb;49(2):836-9
pubmed: 15673783
BMC Bioinformatics. 2010 Mar 08;11:119
pubmed: 20211023
Health Aff (Millwood). 2018 Apr;37(4):662-669
pubmed: 29561692
Environ Sci Technol. 2013 Sep 17;47(18):10197-205
pubmed: 23919449
PeerJ. 2019 Jul 26;7:e7359
pubmed: 31388474
Front Microbiol. 2021 Oct 11;12:717809
pubmed: 34707579
Environ Sci Technol Lett. 2020 Jun 26;7(9):622-631
pubmed: 37566314
Front Microbiol. 2022 Feb 22;12:798442
pubmed: 35273579
Bioinformatics. 2022 Nov 30;38(23):5315-5316
pubmed: 36218463
Water Res. 2020 Sep 15;183:116088
pubmed: 32622239
Nat Microbiol. 2018 Jul;3(7):836-843
pubmed: 29807988
Bioinformatics. 2016 Feb 15;32(4):605-7
pubmed: 26515820
J Antimicrob Chemother. 2014 Jul;69(7):1785-91
pubmed: 24797064
Sci Total Environ. 2019 Dec 20;697:134023
pubmed: 31479900
Nat Methods. 2014 Nov;11(11):1144-6
pubmed: 25218180
Bioinformatics. 2010 Oct 1;26(19):2460-1
pubmed: 20709691
Pathog Dis. 2014 Aug;71(3):292-301
pubmed: 24376225
Sci Total Environ. 2013 Mar 1;447:345-60
pubmed: 23396083
Sci Total Environ. 2023 Aug 20;887:163781
pubmed: 37149193
Bioinformatics. 2011 Nov 1;27(21):2957-63
pubmed: 21903629
Crit Rev Microbiol. 1989;16(4):253-86
pubmed: 2649316
Trends Microbiol. 2021 May;29(5):458-471
pubmed: 33004259
PLoS One. 2013 Oct 25;8(10):e78906
pubmed: 24205347
Water Res. 2007 Mar;41(5):1143-51
pubmed: 17239919
FEMS Microbiol Ecol. 2016 Mar;92(3):
pubmed: 26832203
Water Res. 2015 Nov 15;85:458-66
pubmed: 26372743
Clin Microbiol Rev. 2009 Jan;22(1):161-82, Table of Contents
pubmed: 19136439
Genome Res. 2017 May;27(5):824-834
pubmed: 28298430
Trends Microbiol. 2018 Mar;26(3):220-228
pubmed: 29033338
Sci Rep. 2021 Jun 16;11(1):12728
pubmed: 34135355
Clin Microbiol Rev. 2011 Jan;24(1):174-92
pubmed: 21233511
Can J Microbiol. 2019 Jan;65(1):34-44
pubmed: 30248271
Pharmaceuticals (Basel). 2021 Jun 03;14(6):
pubmed: 34204872
Endocrinology. 1990 Dec;127(6):2757-62
pubmed: 2249627
PeerJ. 2016 Oct 18;4:e2584
pubmed: 27781170
PLoS One. 2014 Aug 01;9(8):e103705
pubmed: 25084517
Front Microbiol. 2023 Jan 16;13:1100102
pubmed: 36733776
Environ Microbiol Rep. 2014 Dec;6(6):696-704
pubmed: 25360571
Genome Res. 2015 Jul;25(7):1043-55
pubmed: 25977477
Huan Jing Ke Xue. 2023 Jan 8;44(1):169-179
pubmed: 36635805
Lancet Infect Dis. 2019 Jan;19(1):56-66
pubmed: 30409683
Ann N Y Acad Sci. 2015 Sep;1354:12-31
pubmed: 26190223
Pathogens. 2021 Jul 02;10(7):
pubmed: 34357984
Microb Genom. 2023 Jul;9(7):
pubmed: 37486746
J Antimicrob Chemother. 2021 Oct 11;76(11):3059-3061
pubmed: 34406409
Nat Commun. 2022 Dec 1;13(1):7251
pubmed: 36456547
Sci Rep. 2022 Mar 2;12(1):3458
pubmed: 35236881
Sci Total Environ. 2015 Apr 15;512-513:316-325
pubmed: 25634736
Proc Natl Acad Sci U S A. 2017 Jan 17;114(3):E367-E375
pubmed: 28049818
J Glob Infect Dis. 2019 Apr-Jun;11(2):59-62
pubmed: 31198308
Water Res. 2014 Oct 1;62:97-106
pubmed: 24937359
Sci Total Environ. 2016 Dec 1;572:697-712
pubmed: 27542633
FEMS Microbiol Ecol. 2016 Mar;92(3):
pubmed: 26832204
Nat Microbiol. 2017 Apr 25;2:17026
pubmed: 28440278