tRNA anticodon cleavage by target-activated CRISPR-Cas13a effector.

RNA, Transfer / genetics CRISPR-Cas Systems Anticodon / genetics Escherichia coli / genetics Leptotrichia / genetics CRISPR-Associated Proteins / metabolism Bacteriophages / genetics RNA Cleavage

Journal

Science advances
ISSN: 2375-2548
Titre abrégé: Sci Adv
Pays: United States
ID NLM: 101653440

Informations de publication

Date de publication:
26 Apr 2024
Historique:
medline: 24 4 2024
pubmed: 24 4 2024
entrez: 24 4 2024
Statut: ppublish

Résumé

Type VI CRISPR-Cas systems are among the few CRISPR varieties that target exclusively RNA. The CRISPR RNA-guided, sequence-specific binding of target RNAs, such as phage transcripts, activates the type VI effector, Cas13. Once activated, Cas13 causes collateral RNA cleavage, which induces bacterial cell dormancy, thus protecting the host population from the phage spread. We show here that the principal form of collateral RNA degradation elicited by

Identifiants

DOI: 10.1126/sciadv.adl0164 PMID: 38657076
pubmed: 38657076
doi: 10.1126/sciadv.adl0164
doi:

Substances chimiques

RNA, Transfer 9014-25-9
Anticodon 0
CRISPR-Associated Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

eadl0164

Auteurs

Ishita Jain (I)

Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
ORCID: 0000-0001-5024-1265

Matvey Kolesnik (M)

Center for Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, Moscow, Russia.
ORCID: 0000-0002-7997-657X

Konstantin Kuznedelov (K)

Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
ORCID: 0000-0002-9040-3895

Leonid Minakhin (L)

Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.

Natalia Morozova (N)

Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, Russia.
ORCID: 0000-0002-3001-2593

Anna Shiriaeva (A)

Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, Russia.
Saint Petersburg State University, Saint Petersburg, Russia.
ORCID: 0000-0002-4271-1658

Alexandr Kirillov (A)

Peter the Great St. Petersburg Polytechnic University, Saint Petersburg, Russia.

Sofia Medvedeva (S)

Center for Molecular and Cellular Biology, Skolkovo Institute of Science and Technology, Moscow, Russia.

Alexei Livenskyi (A)

Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Moscow, Russia.
Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia.
ORCID: 0009-0001-2850-9904

Laura Kazieva (L)

Institute of Biomedical Chemistry, Moscow, Russia.
ORCID: 0000-0002-8636-9161

Kira S Makarova (KS)

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health; Bethesda, MD, USA.
ORCID: 0000-0002-8174-2844

Eugene V Koonin (EV)

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health; Bethesda, MD, USA.
ORCID: 0000-0003-3943-8299

Sergei Borukhov (S)

Department of Cell Biology and Neuroscience, Rowan University School of Osteopathic Medicine at Stratford; Stratford, NJ, USA.
ORCID: 0000-0002-3517-3003

Konstantin Severinov (K)

Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
Center for Precision Genome Editing and Genetic Technologies for Biomedicine, Institute of Gene Biology, Moscow, Russia.
ORCID: 0000-0001-9706-450X

Ekaterina Semenova (E)

Waksman Institute for Microbiology, Rutgers, The State University of New Jersey, Piscataway, NJ, USA.
ORCID: 0000-0001-6816-8879

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Classifications MeSH

questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN de transfert › tRNA anticodon cleavage by target-activated...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Épigenèse génétique › Extinction de l'expression des gènes › Systèmes CRISPR-Cas › tRNA anticodon cleavage by target-activated...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Code génétique › Anticodon › tRNA anticodon cleavage by target-activated...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › tRNA anticodon cleavage by target-activated...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Bactéries anaérobies à Gram négatif › Bâtonnets à Gram négatif anaérobies droits, incurvés et spiralés › Leptotrichia › tRNA anticodon cleavage by target-activated...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines associées aux CRISPR › tRNA anticodon cleavage by target-activated...
questionsmedicales.fr › Virus › Bactériophages › tRNA anticodon cleavage by target-activated...
questionsmedicales.fr › Phénomènes génétiques › Clivage de l'ARN › tRNA anticodon cleavage by target-activated...