Modelling Plasmid-Mediated Horizontal Gene Transfer in Biofilms.
Antimicrobial resistence
Biofilms
Free boundary problem
Horizontal gene transfer
Nonlocal PDEs
Plasmid conjugation
Journal
Bulletin of mathematical biology
ISSN: 1522-9602
Titre abrégé: Bull Math Biol
Pays: United States
ID NLM: 0401404
Informations de publication
Date de publication:
25 Apr 2024
25 Apr 2024
Historique:
received:
11
12
2023
accepted:
27
03
2024
medline:
26
4
2024
pubmed:
26
4
2024
entrez:
25
4
2024
Statut:
epublish
Résumé
In this study, we present a mathematical model for plasmid spread in a growing biofilm, formulated as a nonlocal system of partial differential equations in a 1-D free boundary domain. Plasmids are mobile genetic elements able to transfer to different phylotypes, posing a global health problem when they carry antibiotic resistance factors. We model gene transfer regulation influenced by nearby potential receptors to account for recipient-sensing. We also introduce a promotion function to account for trace metal effects on conjugation, based on literature data. The model qualitatively matches experimental results, showing that contaminants like toxic metals and antibiotics promote plasmid persistence by favoring plasmid carriers and stimulating conjugation. Even at higher contaminant concentrations inhibiting conjugation, plasmid spread persists by strongly inhibiting plasmid-free cells. The model also replicates higher plasmid density in biofilm's most active regions.
Identifiants
pubmed: 38664322
doi: 10.1007/s11538-024-01289-x
pii: 10.1007/s11538-024-01289-x
doi:
Substances chimiques
Anti-Bacterial Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
63Subventions
Organisme : H2020 Marie Skłodowska-Curie Actions
ID : 861088
Informations de copyright
© 2024. The Author(s).
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