Organophosphorus Flame Retardant TPP-Induced Human Corneal Epithelial Cell Apoptosis through Caspase-Dependent Mitochondrial Pathway.
Humans
Apoptosis
/ drug effects
Flame Retardants
/ toxicity
Mitochondria
/ drug effects
Epithelium, Corneal
/ drug effects
Membrane Potential, Mitochondrial
/ drug effects
Cell Survival
/ drug effects
Caspases
/ metabolism
Epithelial Cells
/ drug effects
Organophosphates
/ pharmacology
Cells, Cultured
TPP
cell apoptosis
gene expression
human corneal epithelial cells
mitochondrial apoptosis pathway
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
09 Apr 2024
09 Apr 2024
Historique:
received:
05
03
2024
revised:
01
04
2024
accepted:
04
04
2024
medline:
27
4
2024
pubmed:
27
4
2024
entrez:
27
4
2024
Statut:
epublish
Résumé
A widely used organophosphate flame retardant (OPFR), triphenyl phosphate (TPP), is frequently detected in various environmental media and humans. However, there is little known on the human corneal epithelium of health risk when exposed to TPP. In this study, human normal corneal epithelial cells (HCECs) were used to investigate the cell viability, morphology, apoptosis, and mitochondrial membrane potential after they were exposed to TPP, as well as their underlying molecular mechanisms. We found that TPP decreased cell viability in a concentration-dependent manner, with a half maximal inhibitory concentration (IC50) of 220 μM. Furthermore, TPP significantly induced HCEC apoptosis, decreased mitochondrial membrane potential in a dose-dependent manner, and changed the mRNA levels of the apoptosis biomarker genes (
Identifiants
pubmed: 38673741
pii: ijms25084155
doi: 10.3390/ijms25084155
pii:
doi:
Substances chimiques
Flame Retardants
0
triphenyl phosphate
YZE19Z66EA
Caspases
EC 3.4.22.-
Organophosphates
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : the National Natural Science Foundation of China
ID : 42067059, 21906134
Organisme : the Basic Research Program of Yunnan Province
ID : 202201AT070054
Organisme : the Top Young Talents Project of National Forestry and Grassland Administration
ID : 2020132613
Organisme : the Yunnan Thousand Youth Talent Program
ID : YNQR-QNRC-2018-049
Organisme : the Yunnan Innovative Research Team
ID : 202005AE160017