Nucleoside Phosphorylases make N7-xanthosine.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
29 Apr 2024
29 Apr 2024
Historique:
received:
23
10
2023
accepted:
26
03
2024
medline:
30
4
2024
pubmed:
30
4
2024
entrez:
29
4
2024
Statut:
epublish
Résumé
Modern, highly evolved nucleoside-processing enzymes are known to exhibit perfect regioselectivity over the glycosylation of purine nucleobases at N9. We herein report an exception to this paradigm. Wild-type nucleoside phosphorylases also furnish N7-xanthosine, a "non-native" ribosylation regioisomer of xanthosine. This unusual nucleoside possesses several atypical physicochemical properties such as redshifted absorption spectra, a high equilibrium constant of phosphorolysis and low acidity. Ultimately, the biosynthesis of this previously unknown natural product illustrates how even highly evolved, essential enzymes from primary metabolism are imperfect catalysts.
Identifiants
pubmed: 38684649
doi: 10.1038/s41467-024-47287-4
pii: 10.1038/s41467-024-47287-4
doi:
Substances chimiques
xanthosine
BM66HT53C3
nucleoside phosphorylase
EC 2.4.2.-
Xanthines
0
Pentosyltransferases
EC 2.4.2.-
Ribonucleosides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
3625Subventions
Organisme : Deutsche Forschungsgemeinschaft (German Research Foundation)
ID : 492196858
Informations de copyright
© 2024. The Author(s).
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